Difference between revisions of "Part:BBa K5301023"
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===Characterization=== | ===Characterization=== | ||
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| + | We used SDS-PAGE to test whether this pathway is expressed successfully(Figure 1). The molecular weight of SCSdC-mCh[1-10] is 76.3 kDa. And we purified the target protein with a molecular weight of approximately 70-100 kD (Lane 4 - Lane 5), which demonstrated that we had successfully expressed this pathway. | ||
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| + | https://static.igem.wiki/teams/5301/parts/sds-page-result-of-sdycatcher-for-sdycatcher.png | ||
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| + | Figure 1.SDS-PAGE analysis of the express results of SCSdC-mCh[1-10] (containing SdyCatcher).The gel was run at 80 V for 10 minutes and then at 150 V for 20 minutes, followed by staining with Coomassie Brilliant Blue. The molecular weight of SCSdC-mCh[1-10] is 76.3 kDa. | ||
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Revision as of 15:40, 30 September 2024
This pathway expresses SCSdC-mCh[1-10] using T7 expression system.
This pathway expresses SCSdC-mCh[1-10] using T7 expression system.
Usage and Biology
We used this pathway to express SCSdC-mCh[1-10] by using T7 expression system in E.coli. And the expression of SCSdC-mCh[1-10] can be induced by IPTG. For more information on SCSdC-mCh[1-10], go to BBa_K5301005.
Characterization
We used SDS-PAGE to test whether this pathway is expressed successfully(Figure 1). The molecular weight of SCSdC-mCh[1-10] is 76.3 kDa. And we purified the target protein with a molecular weight of approximately 70-100 kD (Lane 4 - Lane 5), which demonstrated that we had successfully expressed this pathway.
Figure 1.SDS-PAGE analysis of the express results of SCSdC-mCh[1-10] (containing SdyCatcher).The gel was run at 80 V for 10 minutes and then at 150 V for 20 minutes, followed by staining with Coomassie Brilliant Blue. The molecular weight of SCSdC-mCh[1-10] is 76.3 kDa.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1764
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1283
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1079
Illegal AgeI site found at 1872 - 1000COMPATIBLE WITH RFC[1000]