Difference between revisions of "Part:BBa K5097010"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K5097010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5097010 SequenceAndFeatures</partinfo>
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This gene sequence has been altered to make the SpeB gene RCF10 compatible. To do this, we removed an internal PstI restriction site, changing the nucleotide at position 318 from a G to an A, without affecting the amino acid sequence.
  
  

Revision as of 17:38, 30 September 2024


SpeB (Agmatinase)

Usage and Biology

This part codes for the SpeB gene from E.coli. The gene codes for agmatine ureohydrolase. The enzyme is responsible for the catabolism of agmatine. It converts agmatine into urea and putrescine, and is the second step in the metabolic pathway that converts arginine into putrescine (Morris., et 1969). The 2024 Oneonta iGEM team designed the sequence for use in a base producing circuit. When co-expressed with SpeA (BBa_K5097010) it will construct the pathway from turning arginine into putrescine. Acidic conditions will be neutralized with the production of putrescine.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 789
    Illegal BamHI site found at 702
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 724
  • 1000
    COMPATIBLE WITH RFC[1000]

This gene sequence has been altered to make the SpeB gene RCF10 compatible. To do this, we removed an internal PstI restriction site, changing the nucleotide at position 318 from a G to an A, without affecting the amino acid sequence.