Difference between revisions of "Part:BBa K5301028"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K5301028 short</partinfo> | <partinfo>BBa_K5301028 short</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This pathway expresses the SpyCatcher-MSP1D1-SpyTag fusion protein (spMSP1D1) under IPTG-induced conditions, achieving self-cyclization of MSP1D1 to construct nanodiscs.For more information on spMSP1D1, go to <partinfo>BBa_K5301011</partinfo>. | ||
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Revision as of 12:56, 30 September 2024
spMSP1D1 expression pathway (T7)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 571
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 571
Illegal NheI site found at 139
Illegal NheI site found at 1145 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 571
Illegal BglII site found at 999
Illegal BamHI site found at 172
Illegal XhoI site found at 895 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 571
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 571
- 1000COMPATIBLE WITH RFC[1000]