Difference between revisions of "Part:BBa K5301023"
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This pathway expresses SCSdC-mCh[1-10] using T7 expression system. | This pathway expresses SCSdC-mCh[1-10] using T7 expression system. | ||
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| + | ===Usage and Biology=== | ||
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| + | We used this pathway to express SCSdC-mCh[1-10] by using T7 expression system in <i>E.coli</i>. And the expression of SCSdC-mCh[1-10] can be induced by IPTG. For more information on SCSdC-mCh[1-10], go to <partinfo>BBa_K5301005</partinfo>. | ||
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| + | ===Characterization=== | ||
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Revision as of 15:33, 30 September 2024
This pathway expresses SCSdC-mCh[1-10] using T7 expression system.
This pathway expresses SCSdC-mCh[1-10] using T7 expression system.
Usage and Biology
We used this pathway to express SCSdC-mCh[1-10] by using T7 expression system in E.coli. And the expression of SCSdC-mCh[1-10] can be induced by IPTG. For more information on SCSdC-mCh[1-10], go to BBa_K5301005.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1764
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1283
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1079
Illegal AgeI site found at 1872 - 1000COMPATIBLE WITH RFC[1000]