Difference between revisions of "Part:BBa K5097012"
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RBS B0034 with added spacer sequencers
 
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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Revision as of 12:01, 30 September 2024

RBS BBa_B0034 with spacer nucleotides

Usage and Biology

This part is a modified form of the Anderson Collections RBS B0034. Additional nucleotides have been added to the 5’ and 3’ ends of the RBS, the sequence of which is AAGAGGAGA. The purpose of these additional nucleotides is to mimic the spacing created by the RCF10 assembly scars.

Figure 1: Alignment of RBS BBa_B0034 (top) with BBa_B0034 (bottom). Alignment generated using link=https://www.genome.jp/tools-bin/clustalw.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]