Difference between revisions of "Part:BBa K5301002"

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===Characterization===  
 
===Characterization===  
  
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We used SDS-PAGE to test whether this part worked.
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We used SDS-PAGE to test whether the protein containing SdyTag have been expressed successfully.
 
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Figure 1.This is a test.
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Figure 1.SDS-PAGE analysis of the extraction results of SnCSdT (containing SdyTag).The gel was run at 80 V for 10 minutes and then at 150 V for 20 minutes, followed by staining with Coomassie Brilliant Blue. The molecular weight of SnCSdT-M15-GS is 42.1 kDa.
 
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Revision as of 12:50, 30 September 2024


SdyTag can achieve covalent binding of proteins through Tag-Catcher interaction.

SdyTag is a fibronectin-binding protein. It can cooperate with SdyCatcher to achieve covalent binding of proteins through Tag-Catcher interaction. SdyTag and SdyCatcher can also be used alongside SpyTag-SpyCatcher partners, to demonstrate kinetically controlled directed protein ligation, domain specific protein circularization and macromolecular assembly [1].

Usage and Biology

We devised the fusion expression of SdyTag with spNW15 and SnoopCatcher (namely SnCSdT).We employed SdyTag and SdyCatcher to link two proteins - SCSdC-mCh[1-10] and SnCSdT.

Characterization

We used SDS-PAGE to test whether the protein containing SdyTag have been expressed successfully.

sds-page-result-of-sncsdt-for-sdytag.png

Figure 1.SDS-PAGE analysis of the extraction results of SnCSdT (containing SdyTag).The gel was run at 80 V for 10 minutes and then at 150 V for 20 minutes, followed by staining with Coomassie Brilliant Blue. The molecular weight of SnCSdT-M15-GS is 42.1 kDa.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. ↑ Tan, L.L., S.S. Hoon and F.T. Wong, Kinetic Controlled Tag-Catcher Interactions for Directed Covalent Protein Assembly. PLOS ONE, 2016. 11(10): p. e0165074.