Difference between revisions of "Part:BBa K5301002"
Guanjiaxin (Talk | contribs) (→Usage and Biology) |
Guanjiaxin (Talk | contribs) (→Usage and Biology) |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | We employed | + | We devised the fusion expression of SdyTag with spNW15 and SnoopCatcher (namely SnCSdT).We employed SdyTag and SdyCatcher to link two proteins - SCSdC-mCh[1-10] and SnCSdT. |
===Characterization=== | ===Characterization=== | ||
Revision as of 10:16, 30 September 2024
SdyTag can achieve covalent binding of proteins through Tag-Catcher interaction.
SdyTag is a fibronectin-binding protein. It can cooperate with SdyCatcher to achieve covalent binding of proteins through Tag-Catcher interaction. SdyTag and SdyCatcher can also be used alongside SpyTag-SpyCatcher partners, to demonstrate kinetically controlled directed protein ligation, domain specific protein circularization and macromolecular assembly [1].
Usage and Biology
We devised the fusion expression of SdyTag with spNW15 and SnoopCatcher (namely SnCSdT).We employed SdyTag and SdyCatcher to link two proteins - SCSdC-mCh[1-10] and SnCSdT.
Characterization
We used SDS-PAGE to test whether this part worked.
Figure 1.This is a test.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
- ↑ Tan, L.L., S.S. Hoon and F.T. Wong, Kinetic Controlled Tag-Catcher Interactions for Directed Covalent Protein Assembly. PLOS ONE, 2016. 11(10): p. e0165074.