Difference between revisions of "Part:BBa K5115066"
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__TOC__
 
__TOC__
 
===Introduction===
 
===Introduction===
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Most of the U module is the same with [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067（mineral, F module）], except for that in U module the EP is combined with hoxU and in F module the EP is combined with F module. We design this two parts to compare out which subunit for EP to combine with is more effective. The final exam shows that module F works better than module U. For more details about the module and experiment, please check [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067（mineral, F module）].
+
Most of the U module is the same with [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067（mineral, F module）], except for that in U module the EP is combined with hoxU and in F module the EP is combined with F module. We design these two parts to compare out which subunit for EP to combine with is more effective. The final experiment shows that module F works better than module U. For more details about the module and experiment, please check [https://parts.igem.org/Part:BBa_K5115067 BBa_K5115067（mineral, F module）].
  
  

Revision as of 09:49, 30 September 2024


mineral, U module

contributed by Fudan iGEM 2023

Introduction

Most of the U module is the same with BBa_K5115067（mineral, F module）, except for that in U module the EP is combined with hoxU and in F module the EP is combined with F module. We design these two parts to compare out which subunit for EP to combine with is more effective. The final experiment shows that module F works better than module U. For more details about the module and experiment, please check BBa_K5115067（mineral, F module）.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 208
    Illegal NotI site found at 5126
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 366
    Illegal BglII site found at 5912
    Illegal BglII site found at 6730
    Illegal BglII site found at 7023
    Illegal BglII site found at 15401
    Illegal BglII site found at 15479
    Illegal BamHI site found at 7317
    Illegal XhoI site found at 5357
    Illegal XhoI site found at 7524
    Illegal XhoI site found at 9763
    Illegal XhoI site found at 11592
    Illegal XhoI site found at 12754
    Illegal XhoI site found at 14593
    Illegal XhoI site found at 15409
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5791
    Illegal NgoMIV site found at 6329
    Illegal NgoMIV site found at 6539
    Illegal NgoMIV site found at 6851
    Illegal NgoMIV site found at 7617
    Illegal NgoMIV site found at 8297
    Illegal NgoMIV site found at 10904
    Illegal AgeI site found at 874
    Illegal AgeI site found at 1825
    Illegal AgeI site found at 2506
    Illegal AgeI site found at 3460
    Illegal AgeI site found at 12079
    Illegal AgeI site found at 15362
    Illegal AgeI site found at 16361
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 9417
    Illegal BsaI site found at 15183
    Illegal BsaI site found at 15345
    Illegal BsaI.rc site found at 5960
    Illegal BsaI.rc site found at 6464
    Illegal SapI site found at 266
    Illegal SapI.rc site found at 15294


References