Difference between revisions of "Part:BBa K5415003:Design"
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| − | <h5>Three sets of triple HRE containging oligonucleotide from EP0 promoter</h5> | + | <h5>Three sets of triple HRE containging oligonucleotide from EP0 promoter:</h5> |
<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p> | <p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p> | ||
<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p> | <p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p> | ||
<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p> | <p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p> | ||
<p></p> | <p></p> | ||
| − | <h5>Post EcoRV CUT</h5> | + | <h5>Post EcoRV CUT:</h5> |
<p>gat</p> | <p>gat</p> | ||
<p></p> | <p></p> | ||
| − | <h5>CYC1 promoter from pRL_yEGFP_hc</h5> | + | <h5>CYC1 promoter from pRL_yEGFP_hc:</h5> |
<p>gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat</p> | <p>gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat</p> | ||
<p></p> | <p></p> | ||
| − | <h5>yeCherry</h5> | + | <h5>yeCherry:</h5> |
<p>ATGGTAAGCAAAGGTGAAGAAGATAACATGGCTATTATAAAAGAgTTCATGAGATTTAAGGTTCATATGGAAGGTTCTGTTAACGGGCATGAgTTCGAAATTGAA</p> | <p>ATGGTAAGCAAAGGTGAAGAAGATAACATGGCTATTATAAAAGAgTTCATGAGATTTAAGGTTCATATGGAAGGTTCTGTTAACGGGCATGAgTTCGAAATTGAA</p> | ||
<p>GGTGAAGGTGAAGGTAGACCTTATGAAGGTACACAAACAGCTAAGTTAAAGGTCACAAAAGGTGGTCCCTTGCCATTCGCTTGGGACATTTTGTCTCCACAATTT</p> | <p>GGTGAAGGTGAAGGTAGACCTTATGAAGGTACACAAACAGCTAAGTTAAAGGTCACAAAAGGTGGTCCCTTGCCATTCGCTTGGGACATTTTGTCTCCACAATTT</p> | ||
| Line 19: | Line 19: | ||
<p>GAGGACTACACTATTGTTGAACAATATGAAAGAGCTGAAGGCAGACATTCTACTGGTGGTATGGATGAATTGTACAAGTAA</p> | <p>GAGGACTACACTATTGTTGAACAATATGAAAGAGCTGAAGGCAGACATTCTACTGGTGGTATGGATGAATTGTACAAGTAA</p> | ||
<p></p> | <p></p> | ||
| − | <h5>ADH2 terminator</h5> | + | <h5>ADH2 terminator:</h5> |
<p>gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaagataata</p> | <p>gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaagataata</p> | ||
<p>ttctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttcagcagtgttcgt</p> | <p>ttctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttcagcagtgttcgt</p> | ||
<p>tatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat</p> | <p>tatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat</p> | ||
Revision as of 09:20, 30 September 2024
Contents
Three sets of triple HRE containging oligonucleotide from EP0 promoter:
TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC
TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC
TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC
Post EcoRV CUT:
gat
CYC1 promoter from pRL_yEGFP_hc:
gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat
yeCherry:
ATGGTAAGCAAAGGTGAAGAAGATAACATGGCTATTATAAAAGAgTTCATGAGATTTAAGGTTCATATGGAAGGTTCTGTTAACGGGCATGAgTTCGAAATTGAA
GGTGAAGGTGAAGGTAGACCTTATGAAGGTACACAAACAGCTAAGTTAAAGGTCACAAAAGGTGGTCCCTTGCCATTCGCTTGGGACATTTTGTCTCCACAATTT
ATGTACGGTAGCAAGGCATACGTCAAGCACCCAGCTGACATTCCAGATTATTTGAAATTATCTTTTCCTGAAGGGTTTAAATGGGAAAGAGTTATGAATTTTGAG
GATGGTGGTGTTGTCACTGTCACACAAGACTCCAGTTTACAAGATGGTGAGTTCATCTACAAGGTTAAATTGAGAGGTACTAACTTTCCATCAGACGGTCCTGTT
ATGCAAAAGAAAACTATGGGTTGGGAAGCTAGCTCCGAAAGAATGTATCCTGAAGATGGTGCTTTGAAGGGTGAAATTAAGCAAAGATTGAAATTGAAAGATGGT
GGTCATTATGATGCAGAAGTTAAGACAACGTATAAAGCGAAAAAACCAGTTCAGCTTCCAGGTGCTTATAATGTTAATATTAAGTTGGACATTACCTCTCATAAT
GAGGACTACACTATTGTTGAACAATATGAAAGAGCTGAAGGCAGACATTCTACTGGTGGTATGGATGAATTGTACAAGTAA
ADH2 terminator:
gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaagataata
ttctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttcagcagtgttcgt
tatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat