Difference between revisions of "Part:BBa K5079013"

Revision as of 08:59, 30 September 2024

K45_CP_P95 Switch primers into pET-28a Switch 7 (Fwd)

This is the forward primer for Part:BBa_K5079032. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. The sequence is: ACTTTAAGAAGGAGATATAC atgtcgggaaatcccttgg. 3’ GC Content is 53%, 3' length is 19nt and 3' Tm is 65°C.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5079013 short</partinfo>
-This is the forward primer for Part:BBa_K5079032. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. The sequence is: ACTTTAAGAAGGAGATATAC atgtcgggaaatcccttgg. 3’ GC Content is 53%, 3' length is 19nt and 3' Tm is 66°C.
+This is the forward primer for Part:BBa_K5079032. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. The sequence is: ACTTTAAGAAGGAGATATAC atgtcgggaaatcccttgg. 3’ GC Content is 53%, 3' length is 19nt and 3' Tm is 65°C.
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