Difference between revisions of "Part:BBa K5235000"

 
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This part contains two enzymes linked together. First is BsAld, from <i>Bacillus subtilis</i>, is the first step in theanine synthesis, converting pyruvate to alanine. BsAld first binds with NADH to form an enzyme-cofactor complex. Then, pyruvate binds to the active site of the enzyme-cofactor complex, and NADH transfers its electrons to the pyruvate, reducing the carbonyl group (C=O) to an imine (C=NH). The imine intermediate reacts with ammonium ions to form a Schiff base intermediate, which is then hydrolyzed to produce alanine, releasing NAD&#8314; and water.
 
This part contains two enzymes linked together. First is BsAld, from <i>Bacillus subtilis</i>, is the first step in theanine synthesis, converting pyruvate to alanine. BsAld first binds with NADH to form an enzyme-cofactor complex. Then, pyruvate binds to the active site of the enzyme-cofactor complex, and NADH transfers its electrons to the pyruvate, reducing the carbonyl group (C=O) to an imine (C=NH). The imine intermediate reacts with ammonium ions to form a Schiff base intermediate, which is then hydrolyzed to produce alanine, releasing NAD&#8314; and water.
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CsAlaDC, from *Camellia sinensis*, is the second step in theanine synthesis, converting alanine to ethylamine. CsAlaDC requires the cofactor pyridoxal phosphate (PLP), which is the active form of vitamin B6. Initially, the amino group of alanine forms a Schiff base intermediate with the aldehyde group of PLP. In the Schiff base intermediate, electrons on the &#945;-carboxyl group are redistributed, subsequently removing &#945;-hydrogen as carbon dioxide, forming a stable resonance structure. The resulting carbanion accepts a proton, forming the PLP-bound form of ethylamine. Ethylamine dissociates from PLP, restoring PLP to its original state, and the enzyme can proceed to the next catalytic cycle.
 
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Revision as of 09:00, 30 September 2024


BsAld

This part contains two enzymes linked together. First is BsAld, from Bacillus subtilis, is the first step in theanine synthesis, converting pyruvate to alanine. BsAld first binds with NADH to form an enzyme-cofactor complex. Then, pyruvate binds to the active site of the enzyme-cofactor complex, and NADH transfers its electrons to the pyruvate, reducing the carbonyl group (C=O) to an imine (C=NH). The imine intermediate reacts with ammonium ions to form a Schiff base intermediate, which is then hydrolyzed to produce alanine, releasing NAD⁺ and water.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]