Difference between revisions of "Part:BBa K5301011"

Revision as of 08:36, 30 September 2024

Express the SpyCatcher-MSP1D1-SpyTag fusion protein to achieve self-cyclization of MSP1D1 for constructing nanodiscs, while the 6×His tag is used for subsequent protein purification.

Revision as of 08:31, 30 September 2024 (view source) Owens (Talk \| contribs) ← Older edit		Revision as of 08:36, 30 September 2024 (view source) Owens (Talk \| contribs) Newer edit →
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−	~~This pathway expresses~~ the SpyCatcher-MSP1D1-SpyTag fusion protein ~~under IPTG-induced conditions, achieving~~ self-cyclization of MSP1D1 ~~to construct~~ nanodiscs.	+	Express the SpyCatcher-MSP1D1-SpyTag fusion protein to achieve self-cyclization of MSP1D1 for constructing nanodiscs, while the 6×His tag is used for subsequent protein purification.