Difference between revisions of "Part:BBa K5415002:Design"
Gabriela-c (Talk | contribs) |
Gabriela-c (Talk | contribs) |
||
Line 11: | Line 11: | ||
<h5>yeBFP2</h5> | <h5>yeBFP2</h5> | ||
<p>ATGGTGTCAAAAGGTGAAGAATTGTTCACAGGTGTTGTTCCAATTCTAGTTGAATTAGATGGTGACGTGAATGGTCATAAGTTTTCAGTTAGAGGTGAAGGTGA</p> | <p>ATGGTGTCAAAAGGTGAAGAATTGTTCACAGGTGTTGTTCCAATTCTAGTTGAATTAGATGGTGACGTGAATGGTCATAAGTTTTCAGTTAGAGGTGAAGGTGA</p> | ||
− | <p> | + | <p>AGGTGATGCAACAAATGGTAAATTGACATTGAAGTTTATTTGTACAACCGGTAAGTTGCCAGTTCCGTGGCCAACTCTAGTTACTACATTGTCTCATGGGG</p> |
− | <p> | + | <p>TTCAGTGCTTTGCCAGATATCCAGATCACATGAAACAACATGATTTCTTCAAATCTGCTATGCCAGAAGGTTATGTTCAAGAAAGAACCATTTTTTTTAAA</p> |
− | <p> | + | <p>GATGACGGTACATACAAAACAAGAGCTGAAGTTAAGTTCGAAGGTGATACTTTGGTTAATCGCATAGAATTGAAGGGCGTTGATTTTAAGGAAGATGGTAA</p> |
− | <p> | + | <p>CATCCTCGGTCATAAGCTAGAATACAATTTCAATTCTCATAATATCTATATTATGGCTGTTAAACAAAAGAATGGTATTAAGGTTAATTTCAAAATTAGAC</p> |
<p>ATAATGTCGAAGATGGTTCAGTCCAATTAGCTGATCACTATCAACAAAATACCCCTATTGGTGATGGTCCTGTTTTATTGCCCGATTCTCATTATTTGTCAACT</p> | <p>ATAATGTCGAAGATGGTTCAGTCCAATTAGCTGATCACTATCAACAAAATACCCCTATTGGTGATGGTCCTGTTTTATTGCCCGATTCTCATTATTTGTCAACT</p> | ||
− | <p> | + | <p>CAATCTGTTTTATCAAAAGATCCAAACGAAAAACGTGATCATATGGTTTTGTTGGAgTTCAGAACTGCCGCAGGAATTACTTTAGGTATGGATGAACTATA</p> |
<p>TAAGTAG</p> | <p>TAAGTAG</p> | ||
<p></p> | <p></p> |
Revision as of 08:31, 30 September 2024
Contents
Two sets of triple HRE containing oligonucleotide from EP0 promoter
TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC
TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC
Post-EcoRV CUT
gat
CYC1 promoter from pRL_yEGFP_hc
gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat
yeBFP2
ATGGTGTCAAAAGGTGAAGAATTGTTCACAGGTGTTGTTCCAATTCTAGTTGAATTAGATGGTGACGTGAATGGTCATAAGTTTTCAGTTAGAGGTGAAGGTGA
AGGTGATGCAACAAATGGTAAATTGACATTGAAGTTTATTTGTACAACCGGTAAGTTGCCAGTTCCGTGGCCAACTCTAGTTACTACATTGTCTCATGGGG
TTCAGTGCTTTGCCAGATATCCAGATCACATGAAACAACATGATTTCTTCAAATCTGCTATGCCAGAAGGTTATGTTCAAGAAAGAACCATTTTTTTTAAA
GATGACGGTACATACAAAACAAGAGCTGAAGTTAAGTTCGAAGGTGATACTTTGGTTAATCGCATAGAATTGAAGGGCGTTGATTTTAAGGAAGATGGTAA
CATCCTCGGTCATAAGCTAGAATACAATTTCAATTCTCATAATATCTATATTATGGCTGTTAAACAAAAGAATGGTATTAAGGTTAATTTCAAAATTAGAC
ATAATGTCGAAGATGGTTCAGTCCAATTAGCTGATCACTATCAACAAAATACCCCTATTGGTGATGGTCCTGTTTTATTGCCCGATTCTCATTATTTGTCAACT
CAATCTGTTTTATCAAAAGATCCAAACGAAAAACGTGATCATATGGTTTTGTTGGAgTTCAGAACTGCCGCAGGAATTACTTTAGGTATGGATGAACTATA
TAAGTAG
ADH2 terminator
gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaag
ataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttc
agcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat