Difference between revisions of "Part:BBa K5117021"

Revision as of 08:25, 30 September 2024

PcotYZ

This part contains the regulatory sequence of P_cotYZ, which is a promoter of Bacillus subtilis. P_cotYZ is a sporulation-dependent promoter out of the cotYZ gene cluster of B. subtilis, which allows for gene expression only during sporulation. Information regarding the cotYZ operon can be found on the following databases: SubtiWiki, DBTBS.

In previous studies, this promoter has so far provided the highest activity for spore surface display (Bartels et al. 2018, unpublished data of Elif Öztel).

P_cotYZ only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see Contribution page).

Target organism: Bacillus subtilis

Main purpose of use:: Gene expression and production of fusion proteins (especially for spore surface display)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

Bartels J., López Castellanos S., Radeck J., Mascher T. (2018): Sporobeads: The utilization of the Bacillus subtilis endospore crust as a protein display platform. ACS synthetic biology 7(2), 452-461. https://doi.org/10.1021/acssynbio.7b00285

Öztel, E. & Mascher T. (2024): unpublished data.

@@ Line 5: / Line 5: @@
 This part contains the regulatory sequence of P<sub><i>cotYZ</i></sub>, which is a promoter of <i>Bacillus subtilis</i>.
 P<sub><i>cotYZ</i></sub> is a sporulation-dependent promoter out of the <i>cotYZ</i> gene cluster of <i>B. subtilis</i>, which allows for gene expression only during sporulation.
 Information regarding the <i>cotYZ</i> operon can be found on the following databases:
 <html><a href="https://subtiwiki.uni-goettingen.de/">SubtiWiki</a></html>,