Difference between revisions of "Part:BBa K5079029:Design"

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===Design Notes===
 
===Design Notes===
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z
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This part's insertion site has a free energy of 1.0. The insertion's residue has a SASA of 83%. The residue is somewhat similar; not being conserved. This protein switch has gone through circular permutation, and it has a Catalytic Triad RMSD of 0.048. This switch does require a linker. The Difference of Ä Between KLK6 N-C Termini Distance and Insertion Loop Diameter is 2.9 and the loop distance is 5.2. The linker itself is 3 residues long, composed of "GPG".
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===Source===
 
===Source===
  
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Gene fragment – no source
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===References===
 
===References===

Latest revision as of 02:48, 2 October 2024


Protein Switch 4 (N14__CP_N114)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 83
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 83
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 353
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 83
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 83
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part's insertion site has a free energy of 1.0. The insertion's residue has a SASA of 83%. The residue is somewhat similar; not being conserved. This protein switch has gone through circular permutation, and it has a Catalytic Triad RMSD of 0.048. This switch does require a linker. The Difference of Ä Between KLK6 N-C Termini Distance and Insertion Loop Diameter is 2.9 and the loop distance is 5.2. The linker itself is 3 residues long, composed of "GPG".


Source

References