Difference between revisions of "Part:BBa K5255003"
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<p>Basic parts <b>MLacs1 (<a href="https://parts.igem.org/Part:BBa_K5255003">BBa_K5255003</a>)</b> is a mutant of wild-type Lacs1. Long-chain acyl-coenzyme A (CoA) synthetases (LACSs) activate free fatty acids to acyl-CoA thioesters and play critical roles in fatty acid metabolism. Lacs1 can use DHA, CoA and ATP as substrates to catalyze the synthesis of DHA-CoA, which is the precursor of DHA-PC. MLacs1 sequence was obtained by mutating the active site of Lacs1 and it was introduced into <i>Saccharomyces cerevisiae INVSC1</i> and <i>E.coli BL21 (DE3)</i>. The activity of the enzyme expressed by MLacs1 was verified by <i>Escherichia coli</i> and the product catalyzed by MLacs1, DHA-CoA, was verified by <i>Saccharomyces cerevisiae</i> to ensure the synthesis of DHA-PC and the feasibility of subsequent synthesis of DHA-PC.
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<p>Basic parts <b>MLacs1 (<a href="https://parts.igem.org/Part:BBa_K5255003">BBa_K5255003</a>)</b> is a mutant of wild-type <b>Lacs1 (<a href="https://parts.igem.org/Part:BBa_K5255000">BBa_K5255000</a>)</b>. Long-chain acyl-coenzyme A (CoA) synthetases (LACSs) activate free fatty acids to acyl-CoA thioesters and play critical roles in fatty acid metabolism. Lacs1 can use DHA, CoA and ATP as substrates to catalyze the synthesis of DHA-CoA, which is the precursor of DHA-PC. MLacs1 sequence was obtained by mutating the active site of Lacs1 and it was introduced into <i>Saccharomyces cerevisiae INVSC1</i> and <i>E.coli BL21 (DE3)</i>. The activity of the enzyme expressed by MLacs1 was verified by <i>Escherichia coli</i> and the product catalyzed by MLacs1, DHA-CoA, was verified by <i>Saccharomyces cerevisiae</i> to ensure the synthesis of DHA-PC and the feasibility of subsequent synthesis of DHA-PC.
  
  
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<span class='h3bb'><h3>Sequence and Features</h3></span>
 
<span class='h3bb'><h3>Sequence and Features</h3></span>
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<partinfo>BBa_K5255000 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K5255003 SequenceAndFeatures</partinfo>

Revision as of 08:00, 30 September 2024


MLACS1

Basic parts MLacs1 (BBa_K5255003) is a mutant of wild-type Lacs1 (BBa_K5255000). Long-chain acyl-coenzyme A (CoA) synthetases (LACSs) activate free fatty acids to acyl-CoA thioesters and play critical roles in fatty acid metabolism. Lacs1 can use DHA, CoA and ATP as substrates to catalyze the synthesis of DHA-CoA, which is the precursor of DHA-PC. MLacs1 sequence was obtained by mutating the active site of Lacs1 and it was introduced into Saccharomyces cerevisiae INVSC1 and E.coli BL21 (DE3). The activity of the enzyme expressed by MLacs1 was verified by Escherichia coli and the product catalyzed by MLacs1, DHA-CoA, was verified by Saccharomyces cerevisiae to ensure the synthesis of DHA-PC and the feasibility of subsequent synthesis of DHA-PC.

Figure 1 | MLacs1 generated using homology protein modeling with SWISS-MODEL. The model showed a large N-terminal and a small C-terminal      domain linked by highlighted cyan linker (Asp513-Leu518), with ligand ATP and cofactor Mg2+ residing inbetween the two domains.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]