Difference between revisions of "Part:BBa K5117017:Design"
| Line 8: | Line 8: | ||
===Design Notes=== | ===Design Notes=== | ||
removal of restriction sites which are used for RFC[10] or Type IIS standards, | removal of restriction sites which are used for RFC[10] or Type IIS standards, | ||
| − | |||
exchange of start codon (GTG > ATG) for usage in <i>Bacillus subtilis</i> as host organism | exchange of start codon (GTG > ATG) for usage in <i>Bacillus subtilis</i> as host organism | ||
Latest revision as of 07:03, 30 September 2024
BsRBS-BhBglA
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 579
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
removal of restriction sites which are used for RFC[10] or Type IIS standards,
exchange of start codon (GTG > ATG) for usage in Bacillus subtilis as host organism
Source
Bacillus halodurans