Difference between revisions of "Part:BBa K174017"
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===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 18:56, 21 October 2021

CadA promoter with CzrA binding site

In B. subtilis CadA system works as an efflux system to take the metals out in the cell. It is normally repressed by the CzrA (formely YozA) repressor protein.

When metals such as copper, zinc and arsenic are present inside the cell, they can bind to CzrA and derepress the promoter expressing CadA. Hence metals are taken out by the efflux system.

By utilising the promoter from the CadA system, we created a metal sensor, that responds to at least these three metals.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 166
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]