Difference between revisions of "Part:BBa K5335008"

Figure 1. SpyCatcher-Cry6Aa2-CPPs+SpyTag-6*His(pET28a)
BBa_K5335008 SequenceAndFeatures == Usage and Biology == The SpyCatcher-SpyTag system was modified from the CnaB2 domain of Streptococcus pyogenes fibronectin binding protein. SpyCatcher is an immunoglobulin-like protein with a molecular weight of about 12 kDa, and SpyTag is a short peptide composed of 13 residues that can recognize each other with high affinity and spontaneously form isopeptide bonds.Cell Penetrating Peptides (CPPs), also known as Trojan peptides, are a family of short hydrophobic peptides that can pass through the cell membrane. A variety of cellular transmembrane peptides have been found to facilitate the uptake of various molecules by cells, such as RNA/DNA, peptides, radioisotopes, liposomes, nanoparticles, and protein molecules.In our project, we fused Cry6Aa2 with SpyCatcher and CPPs, attaching it to virus-like particles with SpyTag, releasing it into the soil environment through the cleavage of engineered bacteria, and CPPs helped our insecticidal proteins enter the nematode body for killing.