Difference between revisions of "Part:BBa K5415004:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | ccggtagaggtgtggtcaataagagcgacctcatactatacctgagaaagcaacctgacctacaggaaagagttactcaagaataagaattttcgttttaaaacctaagagtcactttaaaatttg | |
| + | tatacacttattttttttataacttatttaataataaaaatcataaatcataagaaattcgcgctctaGTGGTGATGGTGGTGGTGttctgaaaaggggggaaacatagttagatcaggaaattct | ||
| + | tcattgttgtagctgttgctctgatctcccagcatggacagcatctcctggaagacctcaggctggccaggttgctgtgctggcggttgttgaacatgttgctcactagaacttgaacgatgatga | ||
| + | ggctgctggccctgccactgtggccagacacccacaccctctgctgtccgtgtctggaattgtccggcagtctgtccagtctcaggagcaaagttagatccacgattggtgagactagggtaggca | ||
| + | gcagcaccaggcgatgcagttggggcaccagggagggacatggagctgaaggaggatggagtctgaaagctgcccacaccaaactgggaagtacgagtcttagcagtagcctgggtagccacctgc | ||
| + | tgggcagaaaagcctgagcgggtagtaggggtccaagttggggttgctccttgggtggggttggagtggcgggaaatctgggccaacatctgtcccgcagaagctgatggctggacaatggttaca | ||
| + | ggaggggctaggccactattcctgaaattctctgccggccggggggtaggagggaatgtgttgccctgggagaatagctgttgggtggcagggacagtgctggaggagatgcctttactctgatcc | ||
| + | gcattgatgttgtgatagatttctgaaaatcttggatctctatcctgggcaaataaaccatctgacttctcaaggggcttgctgtgttctggtcctgtggttgtcacaggctgaaccacctgggaa | ||
| + | tgattgtagctggccagtccatctcttcctggtaccatgtccaattctgtttgctgttgctgctgcctgggtgccagctgtcctgagcccatctccaggggtaaattagctgtgggacctagttgt | ||
| + | ggcctctggattgtgttggagagtgtaggccgtggttcttggctagagttcttcacattggtgttggtacagatgatgtactcaatttcatctgagtaagggttctggaaagtaaaggagctggtt | ||
| + | ctcatccagagccattcttggttcttagaccggaaccggaacatgacagacagcacttggccttttaatttcactacctgttggaagctgtctcttagaagctgctggtcttcaggatgacaaaat | ||
| + | tctacaatattctttcctaagagttcctgtggctggtagccaacagtagccacacagcggtgatccacaaaagtgaagataccctcaatgttgtgtcgggagatgaactctgttggttgacaaaca | ||
| + | ttactcatgtctgtacagttgggagaactggttacctgcaatctgccaatggccactaggcaaaacttgcttccctggccagcctctgggtcatcatctgggagggaaacacctgctgggggccag | ||
| + | gccctgatgtagcctgtgcagtggaccaccacgaagtgaggttccccatcctttacagagccaagtccattcctgcatctgttcctcacaaagctcagcctattcacagaaactgggtccacagag | ||
| + | ctactgccacacctcattcggcaaataaacgatctccttgagcccatacacattctcatggaagactgctgaccttcctttttcactgttccagtctttagatccaggatacgccctgtcagggca | ||
| + | ttttctgaagtggaaagctgctcacgaagtttatccacatcatctgggtgcacctgatcatagagtgtgctgccaaaccattcagactgtggctggttcaaaacaggagtcacggagtcagacaca | ||
| + | tacaccaccctgcctgtctcacatgagacaataaacagaaagccatctgctgcctccaagatcaaatgtttcagttcctgatcagtgaggaaagacggcttataggagccatcagtggatgtgttg | ||
| + | ccagttccccgcaaggacttcatgtgagaaactgccatgcgtaagatggttagcttgtctggttttcgagccagggcactacaggtgggtaccatatctgacagttctgtgatgtaggctgtcatc | ||
| + | ttgttccgtcgccgccgttcaatttcactgtgattttccctggcaaaccgctccttatcgttagacatctgatcatcatcacacctcaaaaatttactgttcccttctccatcatcatcaaaatcc | ||
| + | agccctggtcgccgcttaatagccctctggacaatggctcctccaccttggattccaggtccagagtttccagaggcaatggctggacccagtgatggtacatctgatgtcatttcggggttggca | ||
| + | gtagtcgccgccatcgaattttcaaaaattcttactttttttttggatggacgcaaagaagtttaataatcatattacatgtgtgaaattgttatccgctcacaattccacacaacatatctaatc | ||
| + | ttacttatatgttgtggaaatgtaaagagccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccg | ||
| + | agcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactag | ||
| + | cttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaat | ||
| + | taatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgtccctatcagtgatagagatctccctatcagtgatagagatattacttcttattcaaatgtaataaaagt | ||
| + | atcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaaccccggatcctattaaaatggagggcgccggcggcgcgaacgacaagaaaaagataagttctgaacgtcgaaa | ||
| + | agaaaagtctcgtgatgcagccagatctcggcgaagtaaagaatctgaagttttttatgagcttgctcatcagttgccacttccacataatgtgagttcgcatcttgataaggcctctgtgatgag | ||
| + | gcttaccatcagctatttgcgtgtgaggaaacttctggatgctggtgatttggatattgaagatgacatgaaagcacagatgaattgcttttatttgaaagccttggatggttttgttatggttct | ||
| + | cacagatgatggtgacatgatttacatttctgataatgtgaacaaatacatgggattaactcagtttgaactaactggacacagtgtgtttgattttactcatccatgtgaccatgaggaaatgag | ||
| + | agaaatgcttacacacagaaatggccttgtgaaaaagggtaaagaacaaaacacacagcgaagtttttttctcagaatgaagtgtaccctaactagccgaggaagaactatgaacataaagtctgc | ||
| + | aacatggaaggtattgcactgcacaggccacattcacgtatatgataccaacagtaaccaacctcagtgtgggtataagaaaccacctatgacctgcttggtgctgatttgtgaacccattcctca | ||
| + | cccatcaaatattgaaattcctttagatagcaagactttcctcagtcgacacagcctggatatgaaattttcttattgtgatgaaagaattaccgaattgatgggatatgagccagaagaactttt | ||
| + | aggccgctcaatttatgaatattatcatgctttggactctgatcatctgaccaaaactcatcatgatatgtttactaaaggacaagtcaccacaggacagtacaggatgcttgccaaaagaggtgg | ||
| + | atatgtctgggttgaaactcaagcaactgtcatatataacaccaagaactctcaaccacagtgcattgtatgtgtgaattacgttgtgagtggtattattcagcacgacttgattttctcccttca | ||
| + | acaaacagaatgtgtccttaaaccggttgaatcttcagatatgaaaatgactcagctattcaccaaagttgaatcagaagatacaagtagcctctttgacaaactcaagaaggaacctgatgcttt | ||
| + | aactttgctggccccagccgctggagacacaatcatatctttagattttggcagcaacgacacagaaactgatgaccagcaacttgaggaagtaccattatataatgatgtaatgctcccctcacc | ||
| + | caacgaaaaattacagaatataaatttggcaatgtctccattacccaccgctgaaacgccaaagccacttcgaagtagtgctgaccctgcactcaatcaagaagttgcattaaaattagaaccaaa | ||
| + | tccagagtcactggaactttcttttaccatgccccagattcaggatcagacacctagtccttccgatggaagcactagacaaagttcacctgagcctaatagtcccagtgaatattgtttttatgt | ||
| + | ggatagtgatatggtcaatgagttcaagttggaattggtagaaaaactttttgctgaagacacagaagcaaagaacccattttctactcaggacacagatttagacttggagatgttagctcccta | ||
| + | tatcccaatggatgatgacttccagttacgttccttcgatcagttgtcaccattagaaagcagttccgcaagccctgaaagcgcaagtcctcaaagcacagttacagtattccagcagactcaaat | ||
| + | acaagaacctactgctaatgccaccactaccactgccaccactgatgaattaaaaacagtgacaaaagaccgtatggaagacattaaaatattgattgcatctccatctcctacccacatacataa | ||
| + | agaaactacaagtgccacatcatcaccatatagagatactcaaagtcggacagcctcaccaaacagagcaggaaaaggagtcatagaacagacagaaaaatctcatccaagaagccctaacgtgtt | ||
| + | atctgtcgctttgagtcaaagaactacagttcctgaggaagaactaaatccaaagatactagctttgcagaatgctcagagaaagcgaaaaatggaacatgatggttcactttttcaagcagtagg | ||
| + | aattggaacattattacagcagccagacgatcatgcagctactacatcactttcttggaaacgtgtaaaaggatgcaaatctagtgaacagaatggaatggagcaaaagacaattattttaatacc | ||
| + | ctctgatttagcatgtagactgctggggcaatcaatggatgaaagtggattaccacagctgaccagttatgattgtgaagttaatgctcctatacaaggcagcagaaacctactacagggtgaaga | ||
| + | attactcagagctttggatcaagttaacCACCACCACCATCACCACtgagcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatg | ||
| + | acagtgttcgaagtttcacgaataaaagataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatgg | ||
| + | tagcttcagcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat | ||
===Source=== | ===Source=== | ||
Revision as of 20:03, 29 September 2024
HIF1 system for yeast humanisation.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3244
Illegal BglII site found at 3452
Illegal BamHI site found at 3355 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 929
Illegal NgoMIV site found at 3383
Illegal AgeI site found at 2972
Illegal AgeI site found at 4459 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
ccggtagaggtgtggtcaataagagcgacctcatactatacctgagaaagcaacctgacctacaggaaagagttactcaagaataagaattttcgttttaaaacctaagagtcactttaaaatttg tatacacttattttttttataacttatttaataataaaaatcataaatcataagaaattcgcgctctaGTGGTGATGGTGGTGGTGttctgaaaaggggggaaacatagttagatcaggaaattct tcattgttgtagctgttgctctgatctcccagcatggacagcatctcctggaagacctcaggctggccaggttgctgtgctggcggttgttgaacatgttgctcactagaacttgaacgatgatga ggctgctggccctgccactgtggccagacacccacaccctctgctgtccgtgtctggaattgtccggcagtctgtccagtctcaggagcaaagttagatccacgattggtgagactagggtaggca gcagcaccaggcgatgcagttggggcaccagggagggacatggagctgaaggaggatggagtctgaaagctgcccacaccaaactgggaagtacgagtcttagcagtagcctgggtagccacctgc tgggcagaaaagcctgagcgggtagtaggggtccaagttggggttgctccttgggtggggttggagtggcgggaaatctgggccaacatctgtcccgcagaagctgatggctggacaatggttaca ggaggggctaggccactattcctgaaattctctgccggccggggggtaggagggaatgtgttgccctgggagaatagctgttgggtggcagggacagtgctggaggagatgcctttactctgatcc gcattgatgttgtgatagatttctgaaaatcttggatctctatcctgggcaaataaaccatctgacttctcaaggggcttgctgtgttctggtcctgtggttgtcacaggctgaaccacctgggaa tgattgtagctggccagtccatctcttcctggtaccatgtccaattctgtttgctgttgctgctgcctgggtgccagctgtcctgagcccatctccaggggtaaattagctgtgggacctagttgt ggcctctggattgtgttggagagtgtaggccgtggttcttggctagagttcttcacattggtgttggtacagatgatgtactcaatttcatctgagtaagggttctggaaagtaaaggagctggtt ctcatccagagccattcttggttcttagaccggaaccggaacatgacagacagcacttggccttttaatttcactacctgttggaagctgtctcttagaagctgctggtcttcaggatgacaaaat tctacaatattctttcctaagagttcctgtggctggtagccaacagtagccacacagcggtgatccacaaaagtgaagataccctcaatgttgtgtcgggagatgaactctgttggttgacaaaca ttactcatgtctgtacagttgggagaactggttacctgcaatctgccaatggccactaggcaaaacttgcttccctggccagcctctgggtcatcatctgggagggaaacacctgctgggggccag gccctgatgtagcctgtgcagtggaccaccacgaagtgaggttccccatcctttacagagccaagtccattcctgcatctgttcctcacaaagctcagcctattcacagaaactgggtccacagag ctactgccacacctcattcggcaaataaacgatctccttgagcccatacacattctcatggaagactgctgaccttcctttttcactgttccagtctttagatccaggatacgccctgtcagggca ttttctgaagtggaaagctgctcacgaagtttatccacatcatctgggtgcacctgatcatagagtgtgctgccaaaccattcagactgtggctggttcaaaacaggagtcacggagtcagacaca tacaccaccctgcctgtctcacatgagacaataaacagaaagccatctgctgcctccaagatcaaatgtttcagttcctgatcagtgaggaaagacggcttataggagccatcagtggatgtgttg ccagttccccgcaaggacttcatgtgagaaactgccatgcgtaagatggttagcttgtctggttttcgagccagggcactacaggtgggtaccatatctgacagttctgtgatgtaggctgtcatc ttgttccgtcgccgccgttcaatttcactgtgattttccctggcaaaccgctccttatcgttagacatctgatcatcatcacacctcaaaaatttactgttcccttctccatcatcatcaaaatcc agccctggtcgccgcttaatagccctctggacaatggctcctccaccttggattccaggtccagagtttccagaggcaatggctggacccagtgatggtacatctgatgtcatttcggggttggca gtagtcgccgccatcgaattttcaaaaattcttactttttttttggatggacgcaaagaagtttaataatcatattacatgtgtgaaattgttatccgctcacaattccacacaacatatctaatc ttacttatatgttgtggaaatgtaaagagccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccg agcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactag cttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaat taatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgtccctatcagtgatagagatctccctatcagtgatagagatattacttcttattcaaatgtaataaaagt atcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaaccccggatcctattaaaatggagggcgccggcggcgcgaacgacaagaaaaagataagttctgaacgtcgaaa agaaaagtctcgtgatgcagccagatctcggcgaagtaaagaatctgaagttttttatgagcttgctcatcagttgccacttccacataatgtgagttcgcatcttgataaggcctctgtgatgag gcttaccatcagctatttgcgtgtgaggaaacttctggatgctggtgatttggatattgaagatgacatgaaagcacagatgaattgcttttatttgaaagccttggatggttttgttatggttct cacagatgatggtgacatgatttacatttctgataatgtgaacaaatacatgggattaactcagtttgaactaactggacacagtgtgtttgattttactcatccatgtgaccatgaggaaatgag agaaatgcttacacacagaaatggccttgtgaaaaagggtaaagaacaaaacacacagcgaagtttttttctcagaatgaagtgtaccctaactagccgaggaagaactatgaacataaagtctgc aacatggaaggtattgcactgcacaggccacattcacgtatatgataccaacagtaaccaacctcagtgtgggtataagaaaccacctatgacctgcttggtgctgatttgtgaacccattcctca cccatcaaatattgaaattcctttagatagcaagactttcctcagtcgacacagcctggatatgaaattttcttattgtgatgaaagaattaccgaattgatgggatatgagccagaagaactttt aggccgctcaatttatgaatattatcatgctttggactctgatcatctgaccaaaactcatcatgatatgtttactaaaggacaagtcaccacaggacagtacaggatgcttgccaaaagaggtgg atatgtctgggttgaaactcaagcaactgtcatatataacaccaagaactctcaaccacagtgcattgtatgtgtgaattacgttgtgagtggtattattcagcacgacttgattttctcccttca acaaacagaatgtgtccttaaaccggttgaatcttcagatatgaaaatgactcagctattcaccaaagttgaatcagaagatacaagtagcctctttgacaaactcaagaaggaacctgatgcttt aactttgctggccccagccgctggagacacaatcatatctttagattttggcagcaacgacacagaaactgatgaccagcaacttgaggaagtaccattatataatgatgtaatgctcccctcacc caacgaaaaattacagaatataaatttggcaatgtctccattacccaccgctgaaacgccaaagccacttcgaagtagtgctgaccctgcactcaatcaagaagttgcattaaaattagaaccaaa tccagagtcactggaactttcttttaccatgccccagattcaggatcagacacctagtccttccgatggaagcactagacaaagttcacctgagcctaatagtcccagtgaatattgtttttatgt ggatagtgatatggtcaatgagttcaagttggaattggtagaaaaactttttgctgaagacacagaagcaaagaacccattttctactcaggacacagatttagacttggagatgttagctcccta tatcccaatggatgatgacttccagttacgttccttcgatcagttgtcaccattagaaagcagttccgcaagccctgaaagcgcaagtcctcaaagcacagttacagtattccagcagactcaaat acaagaacctactgctaatgccaccactaccactgccaccactgatgaattaaaaacagtgacaaaagaccgtatggaagacattaaaatattgattgcatctccatctcctacccacatacataa agaaactacaagtgccacatcatcaccatatagagatactcaaagtcggacagcctcaccaaacagagcaggaaaaggagtcatagaacagacagaaaaatctcatccaagaagccctaacgtgtt atctgtcgctttgagtcaaagaactacagttcctgaggaagaactaaatccaaagatactagctttgcagaatgctcagagaaagcgaaaaatggaacatgatggttcactttttcaagcagtagg aattggaacattattacagcagccagacgatcatgcagctactacatcactttcttggaaacgtgtaaaaggatgcaaatctagtgaacagaatggaatggagcaaaagacaattattttaatacc ctctgatttagcatgtagactgctggggcaatcaatggatgaaagtggattaccacagctgaccagttatgattgtgaagttaatgctcctatacaaggcagcagaaacctactacagggtgaaga attactcagagctttggatcaagttaacCACCACCACCATCACCACtgagcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatg acagtgttcgaagtttcacgaataaaagataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatgg tagcttcagcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat
Source
We utilised two key iGEM BioBricks: HIF-1 beta and HIF-1 alpha