Difference between revisions of "Part:BBa K5108004"
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<p><a href="https://www.uniprot.org/uniprotkb/P83772/entry" target="blank">Creatinine amidohydrolase</a> (CrnA) is an enzyme that catalyzes the degradation of creatinine into creatine in <i>Pseudomonas putida</i>. | <p><a href="https://www.uniprot.org/uniprotkb/P83772/entry" target="blank">Creatinine amidohydrolase</a> (CrnA) is an enzyme that catalyzes the degradation of creatinine into creatine in <i>Pseudomonas putida</i>. | ||
| − | In the context of our project, the gene encoding this enzyme (<i>crnA</i>) was synthesized in an operon together with the gene for creatine degradation. You can find more information about construct and our results in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009.</a> Figure 1 displays the intermediate step of construction. | + | In the context of our project, the gene encoding this enzyme (<i>crnA</i>) was synthesized in an operon together with the gene for creatine degradation. You can find more information about construct and our results in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009.</a> SDS-PAGE, growth analysis and consumption analysis of creatinine by NMR spectroscopy were performed. Figure 1 displays the intermediate step of construction. |
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Revision as of 06:32, 1 October 2024
Creatinine amidohydrolase form Pseudomonas putida
Creatinine amidohydrolase from Pseudomonas putida
- Contents
- Usage and Biology
- References
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 61
Illegal NgoMIV site found at 663 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 199
Illegal BsaI.rc site found at 547
Usage and Biology
Creatinine amidohydrolase (CrnA) is an enzyme that catalyzes the degradation of creatinine into creatine in Pseudomonas putida. In the context of our project, the gene encoding this enzyme (crnA) was synthesized in an operon together with the gene for creatine degradation. You can find more information about construct and our results in BBa_K5108009. SDS-PAGE, growth analysis and consumption analysis of creatinine by NMR spectroscopy were performed. Figure 1 displays the intermediate step of construction.
