Difference between revisions of "Part:BBa K5108004"
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<p><a href="https://www.uniprot.org/uniprotkb/P83772/entry" target="blank">Creatinine amidohydrolase</a> (CrnA) is an enzyme that catalyzes the degradation of creatinine into creatine in <i>Pseudomonas putida</i>.  
 
<p><a href="https://www.uniprot.org/uniprotkb/P83772/entry" target="blank">Creatinine amidohydrolase</a> (CrnA) is an enzyme that catalyzes the degradation of creatinine into creatine in <i>Pseudomonas putida</i>.  
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In the context of our project, the gene encoding this enzyme (<i>crnA</i>) was synthesized in an operon together with the gene for creatine degradation (Figure 1). You can find more information about construct and our results in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009.</a>  
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In the context of our project, the gene encoding this enzyme (<i>crnA</i>) was synthesized in an operon together with the gene for creatine degradation. You can find more information about construct and our results in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009.</a> Figure 1 displays the intermediate step of construction.
 
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Revision as of 19:26, 29 September 2024

Creatinine amidohydrolase form Pseudomonas putida

Creatinine amidohydrolase from Pseudomonas putida


    Contents
  1. Usage and Biology
  2. References



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
    Illegal NgoMIV site found at 663
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 199
    Illegal BsaI.rc site found at 547

Usage and Biology

Creatinine amidohydrolase (CrnA) is an enzyme that catalyzes the degradation of creatinine into creatine in Pseudomonas putida. In the context of our project, the gene encoding this enzyme (crnA) was synthesized in an operon together with the gene for creatine degradation. You can find more information about construct and our results in BBa_K5108009. Figure 1 displays the intermediate step of construction.




Figure 1: Design of the expression plasmid harboring the crnA gene.


References

  • UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry
  • Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151
    •