Difference between revisions of "Part:BBa K174008:Design"

Latest revision as of 22:33, 21 October 2009

SmtA metallothionein protein with CotC and Gfp fusion

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1234
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1046

Design Notes

This brick can be conveniently integrated to B. subtilis using the homology between cotC fusion and the native copy of cotC on the chromosome. This integration leaves the native cotC intact, resulting in a mixture of native and SmtA fused CotC protein.

Source

The smtA sequence was from E. coli and is at Genbank [http://www.ncbi.nlm.nih.gov/gene/6143825?ordinalpos=3&itool=EntrezSystem2.PEntrez.Gene.Gene_ResultsPanel.Gene_RVDocSum here]

References

Cretì, P., F. Trinchella, et al. "Heavy metal bioaccumulation and metallothionein content in tissues of the sea bream Sparus aurata from three different fish farming systems." Environmental Monitoring and Assessment.
Morby, A. P., J. S. Turner, et al. (1993). SmtB is a metal-dependent repressor of the cyanobacterial metallothionein gene smtA: identification of a Zn inhibited DNA-protein complex. 21: 921-925.
Waldron, K. J. and N. J. Robinson (2009). "How do bacterial cells ensure that metalloproteins get the correct metal?" Nat Rev Micro 7(1): 25-35.

@@ Line 6: / Line 6: @@
 ===Design Notes===
-It can be integrated to ''B. subtilis'' using the homology between ''cotC'' fusion and the native copy of ''cotC'' on the chromosome.
+This brick can be conveniently integrated to ''B. subtilis'' using the homology between ''cotC'' fusion and the native copy of ''cotC'' on the chromosome. This integration leaves the native cotC intact, resulting in a mixture of native and SmtA fused CotC protein.
 ===Source===