Difference between revisions of "Part:BBa K243035"
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===Usage and Biology===
 
===Usage and Biology===
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The vector is used for expression of complex molecules in order not to overburden the expressing cells. Also, it is used for proteins that tend to aggregate as well as for the expression of cell toxic proteins.<br>
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The vector is used for expression of complex molecules in order not to overburden the expressing cells. Also, it is used for proteins that tend to aggregate as well as for the expression of cell toxic proteins. We used this vector containing a resistance for chloramphenicol for the expression of the His-Dig-Split-Fok_a construct(BBa_K243036). The expression of the other heterodimeric Fok construct His-FluA-Split-Fok_i (BBa_K243010) took place in a different vector pEx with ampicillin resistance.  <br>
  
  

Revision as of 22:32, 21 October 2009

pJS419

The pJS419 vector is used as an expression vector. It has a weak Shine-Dalgano sequence that enables low expression level of the protein, which can be useful for proteins with a complex stucture or even toxic proteins.

Pjs419.tif

Usage and Biology

The vector is used for expression of complex molecules in order not to overburden the expressing cells. Also, it is used for proteins that tend to aggregate as well as for the expression of cell toxic proteins. We used this vector containing a resistance for chloramphenicol for the expression of the His-Dig-Split-Fok_a construct(BBa_K243036). The expression of the other heterodimeric Fok construct His-FluA-Split-Fok_i (BBa_K243010) took place in a different vector pEx with ampicillin resistance.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2266
    Illegal XbaI site found at 1382
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2267
    Illegal PstI site found at 2281
    Illegal NotI site found at 2274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1494
    Illegal BamHI site found at 2365
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2267
    Illegal XbaI site found at 1382
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal suffix found in sequence at 2257
    Illegal XbaI site found at 1382
    Illegal NgoMIV site found at 1390
    Illegal NgoMIV site found at 2498
    Illegal AgeI site found at 3832
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2114