Difference between revisions of "Part:BBa K208045:Design"

(→‎Design Notes)
 
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
GFP with promoter, RBS, and terminator. In pSB1AK3.
+
The lac promoter, RBS, GFP, and Terminator BioBricks were ligated together to make this composite part. This part is in plasmid pSB1AK3
  
 
===Source===
 
===Source===
  
−
Composite
+
This part was constructed by performing several step-wise ligations of parts that were either already present in the registry (BBa_B0015) or recently added (as of 2009).
  
 
===References===
 
===References===
 +
Please see the original pages for these Biobricks for more details on references and Silver-fusion.

Latest revision as of 15:59, 22 October 2009

Lac Promoter/RBS/GFP/Term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 230
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 749
    Illegal XhoI site found at 650
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The lac promoter, RBS, GFP, and Terminator BioBricks were ligated together to make this composite part. This part is in plasmid pSB1AK3

Source

This part was constructed by performing several step-wise ligations of parts that were either already present in the registry (BBa_B0015) or recently added (as of 2009).

References

Please see the original pages for these Biobricks for more details on references and Silver-fusion.