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Revision as of 13:10, 29 September 2024


Creatinine amidohydrolase form Pseudomonas putida


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
    Illegal NgoMIV site found at 663
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 199
    Illegal BsaI.rc site found at 547


Sources

The codon optimized nucleotidic sequence from Pseudomonas putida adapted to Pseudomonas putida is obtained thanks to the Codon Optimization Tool from Integrated DNA Technologies codon optimizer tool.. This step permits to lower complexity and minimize secondary structures. The Part was obtained through IDT gBlock synthesis

References

  • UniProt. (s. d.). https://www.uniprot.org/uniprotkb/P83772/entry
  • Tsuru, D., Oka, I., & Yoshimoto, T. (1976c). Creatinine Decomposing Enzymes inPseudomonas putida. Agricultural And Biological Chemistry, 40(5), 1011‑1018. https://doi.org/10.1080/00021369.1976.10862151