Difference between revisions of "Part:BBa K5322003"

 
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===Usage and Biology===
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==Usage and Biology==
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<p>
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Plasm~~~~~~
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==Construction of the plasmid==
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5322003 SequenceAndFeatures</partinfo>
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<p>
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In o3119-``````````
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<img src="https://static.igem.wiki/teams/5322/wet-lab/20-pet29a-j23119-rbs-mfp3-t7.png" alt="pET29a-J23119-RBS-Mfp3-T7" width="300">
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<p align="center"><b>Figure 2-1</b>  Plasmid pET29(a)-p<i>J23119-SoxR</i>-T-p<i>SoxS</i>-RBS-<i>Mfp3</i>-T7</p>
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<img src="https://static.igem.wiki/teams/5101/partpage/phix174e-gel.png" alt="gel" width="500">
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<p align="center"><b>Figure 2-2</b>  Colony PCR gel electrophoresis of plasmid pET29(a)-p<i>J23119-SoxR</i>-T-p<i>SoxS</i>-RBS-PhiX174E-T7(1320bp)</p>
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<img src="https://static.igem.wiki/teams/5101/partpage/phix174e.png" alt="cexu" width="600">
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<p align="center"><b>Figure 2-3</b>  plasmid pET29(a)-p<i>J23119-SoxR</i>-T-p<i>SoxS</i>-RBS-PhiX174E-T7 sequencing result</p>
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==Protein Expression Validation==
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We verified the performance of the lysis module through dry experiments and plan to complete wet experimental validation of its lytic function in the future. Through literature review and numerical modeling validation, we found that after inducing the expression of antimicrobial peptides and lysis proteins with NO for twenty minutes, the engineered strain will be lysed by the lysis protein and release the antimicrobial peptides. Mathematical modeling confirmed that at this time, the concentration of antimicrobial peptides is sufficient to reach an effective inhibitory concentration.
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</html>
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==Sequence and Features==
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<partinfo>BBa_K5101004 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
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==Functional Parameters==
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<partinfo>BBa_K5322003 parameters</partinfo>
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<partinfo>BBa_K5101004 parameters</partinfo>
 
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Revision as of 11:21, 29 September 2024


Constitutive Mfp53 Expression System

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