Difference between revisions of "Part:BBa K5322002"

Revision as of 11:18, 29 September 2024

Constitutive Mfp5 Expression System

Nitric-oxide-inducible lysis module

Usage and Biology

Plasm11:14, 29 September 2024 (UTC)~

Construction of the plasmid

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Figure 2-1 Plasmid pET29(a)-pJ23119-SoxR-T-pSoxS-RBS-Mfp3-T7

Figure 2-2 Colony PCR gel electrophoresis of plasmid pET29(a)-pJ23119-SoxR-T-pSoxS-RBS-PhiX174E-T7(1320bp)

Figure 2-3 plasmid pET29(a)-pJ23119-SoxR-T-pSoxS-RBS-PhiX174E-T7 sequencing result

Protein Expression Validation

We verified the performance of the lysis module through dry experiments and plan to complete wet experimental validation of its lytic function in the future. Through literature review and numerical modeling validation, we found that after inducing the expression of antimicrobial peptides and lysis proteins with NO for twenty minutes, the engineered strain will be lysed by the lysis protein and release the antimicrobial peptides. Mathematical modeling confirmed that at this time, the concentration of antimicrobial peptides is sufficient to reach an effective inhibitory concentration.

Sequence and Features