Difference between revisions of "Part:BBa K5311006"

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<p><strong>Advantages of Endolysins Against <em>C. acnes</em></strong>:</p>
 
<p><strong>Advantages of Endolysins Against <em>C. acnes</em></strong>:</p>
 
<ul>
 
<ul>
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   <li><strong>High Specificity:</strong> Endolysins are typically highly specific to their target bacteria, minimizing the risk of disrupting the beneficial skin microbiota.</li>
+
   <li><strong>High Specificity:</strong> The CAP 3-10 Endolysin is known for its high specificity towards C. acnes, often recognizing unique peptidoglycan motifs present in the bacterial cell wall. This ensures that they selectively lyse the pathogenic bacteria without affecting other beneficial members of the skin microbiota [2]. </li>
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   <li><strong>Low Risk of Resistance:</strong> Unlike traditional antibiotics, endolysins act on essential and conserved structural components of the bacterial cell wall, making it difficult for bacteria to develop resistance.</li>
+
   <li><strong>Low Risk of Resistance:</strong> Unlike traditional antibiotics, which typically target essential processes such as protein synthesis or DNA replication, endolysins act on the structural components of the bacterial cell wall, such as the peptidoglycan layer. The peptidoglycan is a highly conserved and essential component that is less prone to mutation, reducing the likelihood of bacteria developing resistance [3].</li>
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   <li><strong>Direct Bacterial Destruction:</strong> In contrast to other treatments that only inhibit bacterial growth, endolysins cause direct and rapid bacterial cell death, making them a potent tool for treating <em>C. acnes</em>-associated infections.</li>
+
   <li><strong>Safety and reduced side effects:</strong> Endolysins have a favorable safety profile compared to traditional antibiotics, as they are proteins that are rapidly degraded in the human body without inducing toxic side effects. Furthermore, since they specifically target bacterial cell wall components, they do not interact with human cells, making them safe for topical or systemic applications [4].</li>
 
</ul>
 
</ul>

Revision as of 10:22, 29 September 2024

CAP 10-3 Endolysin


Sequencing

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 266
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 34
    Illegal NgoMIV site found at 445
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 193

Biology

In the case of the endolysin derived from the CAP 10-3 bacteriophage, its action specifically targets the degradation of the cell wall of Cutibacterium acnes, a Gram-positive bacterium with a cell wall rich in peptidoglycan, which is a crucial structural component. This endolysin is classified as an N-acetylmuramoyl-L-alanine amidase, which cleaves the bond between the N-acetylmuramic acid and the L-alanine residue in the peptidoglycan backbone.

By breaking down the peptidoglycan, the endolysin disrupts the rigid structure of the cell wall, leading to a loss of osmotic integrity. As a result, the bacterial cell can no longer withstand the internal pressure, causing rapid cell lysis. This mechanism is particularly effective against Gram-positive bacteria like C. acnes because they have a thick peptidoglycan layer that is more accessible to endolysins, unlike Gram-negative bacteria, which possess an additional outer membrane that restricts endolysin access.[1]

Advantages of Endolysins Against C. acnes:

  • High Specificity: The CAP 3-10 Endolysin is known for its high specificity towards C. acnes, often recognizing unique peptidoglycan motifs present in the bacterial cell wall. This ensures that they selectively lyse the pathogenic bacteria without affecting other beneficial members of the skin microbiota [2].
  • Low Risk of Resistance: Unlike traditional antibiotics, which typically target essential processes such as protein synthesis or DNA replication, endolysins act on the structural components of the bacterial cell wall, such as the peptidoglycan layer. The peptidoglycan is a highly conserved and essential component that is less prone to mutation, reducing the likelihood of bacteria developing resistance [3].
  • Safety and reduced side effects: Endolysins have a favorable safety profile compared to traditional antibiotics, as they are proteins that are rapidly degraded in the human body without inducing toxic side effects. Furthermore, since they specifically target bacterial cell wall components, they do not interact with human cells, making them safe for topical or systemic applications [4].