Difference between revisions of "Part:BBa K5269025"
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<partinfo>BBa_K5269025 short</partinfo> | <partinfo>BBa_K5269025 short</partinfo> | ||
| − | This sequence contains T7 promoter, GFP targeted shRNA(BBa_K5269019), T7 terminator and Golden Gate Assembly Site(GGA Site) which includes tac promoter, BsmB I insert (BBa_K5269032), and L3S2P21 terminator as the Golden Gate Assembly Site (GGA Site). The GGA Site used in this sequence has the same basic structure as BBa_K5269020, but the sequence is slightly different. This sequence is a linearized pBluescript II SK(-) In-Fusion reverse primer 2 (BBa_K5269028) and pBluescript II SK(-) In-Fusion forward primer 2 (BBa_K5269029). In-Fusion to pBluescript II SK(-). This allows for shRNA production and Golden Gate Assembly of Surface displayed protein. | + | This sequence contains T7 promoter, GFP targeted shRNA(BBa_K5269019), T7 terminator and Golden Gate Assembly Site(GGA Site) which includes tac promoter, BsmB I insert (BBa_K5269032), and L3S2P21 terminator as the Golden Gate Assembly Site (GGA Site). The GGA Site used in this sequence has the same basic structure as BBa_K5269020, but the sequence is slightly different. This sequence is a linearized pBluescript II SK(-) In-Fusion reverse primer 2 (BBa_K5269028) and pBluescript II SK(-) In-Fusion forward primer 2 (BBa_K5269029). In-Fusion to pBluescript II SK(-). This allows for shRNA production and Golden Gate Assembly of Surface displayed protein. Here we show an image of this shRNA expressed in E. coli, reverse-transcribed and detected by electrophoresis. |
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| + | <html> | ||
| + | <img src="https://static.igem.wiki/teams/5269/parts-registry/shrna.webp" alt="SDS-PAGE Image" style="width:10%;> | ||
| + | <br> | ||
| + | </html> | ||
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| − | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'><br><br>Sequence and Features</span> |
<partinfo>BBa_K5269025 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5269025 SequenceAndFeatures</partinfo> | ||
Latest revision as of 13:28, 2 October 2024
T7 promoter-shRNA-T7 terminator-Golden Gate Assembly Site
This sequence contains T7 promoter, GFP targeted shRNA(BBa_K5269019), T7 terminator and Golden Gate Assembly Site(GGA Site) which includes tac promoter, BsmB I insert (BBa_K5269032), and L3S2P21 terminator as the Golden Gate Assembly Site (GGA Site). The GGA Site used in this sequence has the same basic structure as BBa_K5269020, but the sequence is slightly different. This sequence is a linearized pBluescript II SK(-) In-Fusion reverse primer 2 (BBa_K5269028) and pBluescript II SK(-) In-Fusion forward primer 2 (BBa_K5269029). In-Fusion to pBluescript II SK(-). This allows for shRNA production and Golden Gate Assembly of Surface displayed protein. Here we show an image of this shRNA expressed in E. coli, reverse-transcribed and detected by electrophoresis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 315
Illegal BamHI site found at 420 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]