Difference between revisions of "Part:BBa K5507008:Experience"

 
 
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a. Construction of BBa_K5507008
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For the overexpression of the pgi gene from E. coli, the sacB locus, which encodes levansucrase, was selected as a homologous recombination site because knocking out this gene has no metabolic effects under glucose conditions. First, the kana expression cassette will replace the sacB gene locus as a selectable marker. Then, pgi will replace the kana gene, resulting in the loss of kana resistance, confirming successful gene insertion.
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<center>< img src = "https://static.igem.wiki/teams/5507/parts-figures/hw-figure-1.png" style = "width:500px"></center>
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<figcaption><center> Figure 1. Gene map of BBa_K5507008</center></figcaption>
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Latest revision as of 09:04, 29 September 2024


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K5507008

User Reviews

UNIQ7325bed64a7d6b9d-partinfo-00000000-QINU UNIQ7325bed64a7d6b9d-partinfo-00000001-QINU

a. Construction of BBa_K5507008 For the overexpression of the pgi gene from E. coli, the sacB locus, which encodes levansucrase, was selected as a homologous recombination site because knocking out this gene has no metabolic effects under glucose conditions. First, the kana expression cassette will replace the sacB gene locus as a selectable marker. Then, pgi will replace the kana gene, resulting in the loss of kana resistance, confirming successful gene insertion.

< img src = "https://static.igem.wiki/teams/5507/parts-figures/hw-figure-1.png" style = "width:500px">
Figure 1. Gene map of BBa_K5507008