Difference between revisions of "Part:BBa K180010"
 
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Composite part, consisting of BBa_K180011 and BBa_K180012, but with BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand.
 
Composite part, consisting of BBa_K180011 and BBa_K180012, but with BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand.
  
−
BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180011) has no effect over mCherry or LasI expression in BBa_K180012.
+
BBa_K180011 reads along reverse strand, and BBa_K180012 reads along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180011) has no effect over mCherry or LasI expression in BBa_K180012.
  
 
mCherry and LasI are generated under regulation by tac pR, whilst RhlR is produced constitutively.
 
mCherry and LasI are generated under regulation by tac pR, whilst RhlR is produced constitutively.

Latest revision as of 21:24, 21 October 2009

Rock-paper-scissors - Rock primary plasmid

Composite part, consisting of BBa_K180011 and BBa_K180012, but with BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand.

BBa_K180011 reads along reverse strand, and BBa_K180012 reads along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180011) has no effect over mCherry or LasI expression in BBa_K180012.

mCherry and LasI are generated under regulation by tac pR, whilst RhlR is produced constitutively.

Forms the 'rock' primary plasmid in Team:Southampton's rock-paper-scissors project design.


Usage and Biology

This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).

To function as the 'Rock' in the rock-paper-scissors design by Team:Southampton, the part has to be used in conjunction with K180013, and pairs K180014 & K180016, K180017 & K180020.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2760
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 893
    Illegal NheI site found at 916
    Illegal PstI site found at 2760
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 622
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2760
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2760
    Illegal AgeI site found at 3365
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 147