Difference between revisions of "Part:BBa K5291028"
 
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NadM encoded by <i>nadM</i> is involved in both de novo and salvage pathways of NAD biosynthesis. Bifunctional nicotinamide-nucleotide adenylyltransferase catalyzes the addition of adenylate from ATP to &#946;-nicotinamide D-ribonucleotide, forming NAD+. We used the <i>nadM</i> gene to maintain intracellular NAD+ levels.
 
NadM encoded by <i>nadM</i> is involved in both de novo and salvage pathways of NAD biosynthesis. Bifunctional nicotinamide-nucleotide adenylyltransferase catalyzes the addition of adenylate from ATP to &#946;-nicotinamide D-ribonucleotide, forming NAD+. We used the <i>nadM</i> gene to maintain intracellular NAD+ levels.
  
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===Usage and Biology===
 
===Usage and Biology===
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In carbon fixation module we constructed pBBR1MCS2-<i>nadM-nadK-pntA-pntB</i> to improve the intracellular NADPH and NADP<sup>+</sup> level.<br>
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<html><img width = "600" src="https://static.igem.wiki/teams/5291/images/parts-lhs/pbbr1mcs2-pnta-pntb-nadm-nadk.png" /></html><br>
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<b>Fig.1 The map of plasmid pBBR1MCS2-<i>nadM-nadK-pntA-pntB</i>.</b><br><br>
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We successfully cloned the <i>nadM-nadK</i> gene by PCR.<br>
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<html><img width = "300" src="https://static.igem.wiki/teams/5291/images/result/electropherogram-of-nadm-nadk-pcr-gel.png" /></html><html><img width = "500" src="https://static.igem.wiki/teams/5291/images/parts-lhs/nadm-nadk-pnta-pntb.png" /></html><br>
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<b>Fig.2 Electropherogram of <i>nadM-nadK</i> PCR gel.</b>
  
 
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Latest revision as of 00:57, 2 October 2024


nadM: bifunctional nicotinamide-nucleotide adenylyltransferase

NadM encoded by nadM is involved in both de novo and salvage pathways of NAD biosynthesis. Bifunctional nicotinamide-nucleotide adenylyltransferase catalyzes the addition of adenylate from ATP to β-nicotinamide D-ribonucleotide, forming NAD+. We used the nadM gene to maintain intracellular NAD+ levels.


Usage and Biology

In carbon fixation module we constructed pBBR1MCS2-nadM-nadK-pntA-pntB to improve the intracellular NADPH and NADP+ level.

Fig.1 The map of plasmid pBBR1MCS2-nadM-nadK-pntA-pntB.

We successfully cloned the nadM-nadK gene by PCR.

Fig.2 Electropherogram of nadM-nadK PCR gel.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]