Difference between revisions of "Part:BBa K180010"

Revision as of 21:23, 21 October 2009

Rock-paper-scissors - Rock primary plasmid

Composite part, consisting of BBa_K180011 and BBa_K180012, but with BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand.

BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180011) has no effect over mCherry or LasI expression in BBa_K180012.

mCherry and LasI are generated under regulation by tac pR, whilst RhlR is produced constitutively.

Forms the 'rock' primary plasmid in Team:Southampton's rock-paper-scissors project design.

Usage and Biology

This part has been specified as a basic BioBrick, due to some of the component parts reading in reverse (unable to specify using the component BioBrick format).

To function as the 'Rock' in the rock-paper-scissors design by Team:Southampton, the part has to be used in conjunction with K180013, and pairs K180014 & K180016, K180017 & K180020.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 2760
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 893
Illegal NheI site found at 916
Illegal PstI site found at 2760
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 622
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 2760
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 2760
Illegal AgeI site found at 3365
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 147

@@ Line 5: / Line 5: @@
 BBa_K180011 reading along reverse strand, and BBa_K180012 reading along the forward strand to ensure the constitutive promoter BBa_J23119 (present in BBa_K180011) has no effect over mCherry or LasI expression in BBa_K180012.
+mCherry and LasI are generated under regulation by tac pR, whilst RhlR is produced constitutively.
 Forms the 'rock' primary plasmid in Team:Southampton's rock-paper-scissors project design.