Difference between revisions of "Part:BBa K209473"
 
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===Usage and Biology===
 
===Usage and Biology===
 
pcDNA3.1(+) is a vector designed for high-level, constitutive expression in a variety of mammalian cell lines.
 
pcDNA3.1(+) is a vector designed for high-level, constitutive expression in a variety of mammalian cell lines.
 +
 +
===Documentation and characterization===
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This was the standard expression vector for all constructs described in the following experiments: 
 +
 +
http://2009.igem.org/Team:UCSF/Navigation
 +
 +
http://2009.igem.org/Team:UCSF/NavigationPart2
 +
 +
 +
  
 
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Latest revision as of 21:22, 1 November 2009

pcDNA3.1(+) with AarI A-D sites in MCS

This is an expression vector backbone, in which AarI A-D sites have been inserted into the MCS.


Usage and Biology

pcDNA3.1(+) is a vector designed for high-level, constitutive expression in a variety of mammalian cell lines.

Documentation and characterization

This was the standard expression vector for all constructs described in the following experiments:

http://2009.igem.org/Team:UCSF/Navigation

http://2009.igem.org/Team:UCSF/NavigationPart2



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2387
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 952
    Illegal NheI site found at 895
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2387
    Illegal NotI site found at 978
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 952
    Illegal BglII site found at 12
    Illegal BamHI site found at 929
    Illegal XhoI site found at 1059
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2387
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 952
    Illegal XbaI site found at 1065
    Illegal SpeI site found at 249
    Illegal SpeI site found at 935
    Illegal PstI site found at 957
    Illegal PstI site found at 2387
    Illegal NgoMIV site found at 1497
    Illegal NgoMIV site found at 2838
    Illegal NgoMIV site found at 3121
    Illegal AgeI site found at 1013
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 882
    Illegal BsaI.rc site found at 4646
    Illegal SapI site found at 3566
    Illegal SapI.rc site found at 2687
    Illegal SapI.rc site found at 2897