Difference between revisions of "Part:BBa K5335006"
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By adding His tag to the end of Cry6Aa2, we constructed an expression and purification vector of Cry6Aa2 on plasmid pET28a and transformed the constructed vector into Escherichia coli BL21(DE3). | By adding His tag to the end of Cry6Aa2, we constructed an expression and purification vector of Cry6Aa2 on plasmid pET28a and transformed the constructed vector into Escherichia coli BL21(DE3). | ||
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Revision as of 05:35, 29 September 2024
Cry6Aa2 is a Nematocidal crystal protein from Bacillus thuringiensis YBT-1518
Cry6Aa2 is a pesticidal crystal protein that mainly in the gut of nematodes by triggering a specific necrosis pathway different from other Cry proteins.Total structure weight of Cry6Aa2 protein is 48.23 kDa.Cry6Aa2 can be expressed in E. coli BL21 (DE3) and exert its toxicity.
Usage and Biology
As a Gram-positive bacterium, Bacillus thuringiensis produces a variety of insecticidal crystal proteins during its growth. In the past hundred years, Bacillus thuringiensis has been widely developed and studied as a microbial insecticidal agent. The gene encoding the insecticidal crystal protein is the main source of transgenic insect-resistant plants. Cry6Aa2 is a crystal protein from Bt YBT-1518 that kills nematodes through a specific necrotic pathway. In our project, we fused Cry6Aa2 with SpyCatcher, attached it to virus-like particles with Spytag, and released it into the soil environment to kill nematodes through the cleavage of engineered bacteria.
