Difference between revisions of "Part:BBa K243008:Design"
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The middle length of the linker is useful to produce a stable connection to another part and to create a certain distance. The middle distance produced by the linker reduces possible sterical interferences and increases the distance from the protein Fok_a to the DNA when it is linked to a DNA binding protein. We had to prove the choice of distance between two parts and the distance between our protein domains Fok_a and Fok_i. <br> | The middle length of the linker is useful to produce a stable connection to another part and to create a certain distance. The middle distance produced by the linker reduces possible sterical interferences and increases the distance from the protein Fok_a to the DNA when it is linked to a DNA binding protein. We had to prove the choice of distance between two parts and the distance between our protein domains Fok_a and Fok_i. <br> | ||
Designed according to the [https://parts.igem.org/Assembly_standard_25 RFC 25]. | Designed according to the [https://parts.igem.org/Assembly_standard_25 RFC 25]. | ||
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| + | [https://static.igem.org/mediawiki/parts/f/f3/Freiburg09_ML-FokA.txt Commented GenBank file] | ||
===Source=== | ===Source=== | ||
Latest revision as of 00:53, 22 October 2009
Middle Linker-Fok_a
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 517
Design Notes
The middle length of the linker is useful to produce a stable connection to another part and to create a certain distance. The middle distance produced by the linker reduces possible sterical interferences and increases the distance from the protein Fok_a to the DNA when it is linked to a DNA binding protein. We had to prove the choice of distance between two parts and the distance between our protein domains Fok_a and Fok_i.
Designed according to the RFC 25.
Source
Combinated by cloning.