Difference between revisions of "Part:BBa K5269022"

Revision as of 08:22, 30 September 2024

SpyTag-GH19Chitinase-His-Tag and BsmB I cut site(for GGA)

This sequence contains SD-sequence(BBa_K5269034), SpyTag, GH19Chitinase (BBa_K5269015) and His-Tag sequence. In addition, the BsmB I cleavage sites at both ends of the sequence allow for Golden Gate Assembly to the Golden Gate Assembly Site (BBa_K5269020) and T7 promoter-shRNA-T7 terminator-Golden Gate Assembly Site(BBa_K5269025). The SpyCatcher-SpyTag system works when used with Lpp-OmpA-SpyCatcher(BBa_K5269026) or INPNC-SpyCatcher(BBa_K5269027). Therefore, GH19Chitinase, which hydrolyzes chitin contained in the target bacterial membrane, is expected to be stably present on the Membrane Vesicle, which will facilitate the introduction of shRNA into the target fungus cells.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 20
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 356
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 885
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 20
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 20
Illegal NgoMIV site found at 686
Illegal AgeI site found at 641
1000
COMPATIBLE WITH RFC[1000]

Revision as of 03:46, 29 September 2024 (view source) Nakaiyuichiro (Talk \| contribs) ← Older edit		Revision as of 08:22, 30 September 2024 (view source) Nakaiyuichiro (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K5269022 short</partinfo>		<partinfo>BBa_K5269022 short</partinfo>

−	This sequence contains SD-sequence(BBa_K5269034), SpyTag, GH19Chitinase (BBa_K5269015) and His-Tag sequence. In addition, the BsmB I cleavage sites at both ends of the sequence allow for Golden Gate Assembly to the Golden Gate Assembly Site (BBa_K5269020) and T7 promoter-shRNA-T7 terminator-Golden Gate Assembly Site(BBa_K5269025). The SpyCatcher-SpyTag system works when used with Lpp-~~ompA~~-SpyCatcher(BBa_K5269026) or INPNC-SpyCatcher(BBa_K5269027). Therefore, GH19Chitinase, which hydrolyzes chitin contained in the target bacterial membrane, is expected to be stably present on the Membrane Vesicle, which will facilitate the introduction of shRNA into the target fungus cells.	+	This sequence contains SD-sequence(BBa_K5269034), SpyTag, GH19Chitinase (BBa_K5269015) and His-Tag sequence. In addition, the BsmB I cleavage sites at both ends of the sequence allow for Golden Gate Assembly to the Golden Gate Assembly Site (BBa_K5269020) and T7 promoter-shRNA-T7 terminator-Golden Gate Assembly Site(BBa_K5269025). The SpyCatcher-SpyTag system works when used with Lpp-OmpA-SpyCatcher(BBa_K5269026) or INPNC-SpyCatcher(BBa_K5269027). Therefore, GH19Chitinase, which hydrolyzes chitin contained in the target bacterial membrane, is expected to be stably present on the Membrane Vesicle, which will facilitate the introduction of shRNA into the target fungus cells.