Difference between revisions of "Part:BBa K208001"
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| − | <partinfo>BBa_K208001 short</partinfo> | + | <partinfo>BBa_K208001 short</partinfo><br> |
| − | + | This is a Silver-fusion compatible BioBrick part that codes for phasin. The Silver-fusion compatibility standard was used so that this part could be fused with small signal peptides, which target proteins for export out of the cytoplasm. This part is functional both in fusion and non-fusion applications. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | <br>Phasin (PhaP) is a low-molecular weight protein that plays a role in PHA granule formation by physically binding to the PHA granule surface (York, 2001). The specific purpose of phasin production is not completely understood (York 2002), although some of the affects of the phasin/PHA interaction have been studied. York et al (2001) determined that the production of phasin is dependent on PHA accumulation. Specifically, it is suggested that phasin expression requires the presence of PHA synthase (York, 2001). Maehara et al (1999) observed that the level of PHA accumulation substantially decreases and the size of PHA granules increases when phasin is either absent or regulated by a repressor, PhaR. Therefore, PHA production levels are enhanced in the presence of phasin due to an increased granule surface-to-volume ratio (York 2001; Maehara 1999).<br> | ||
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| + | The Silver-fusion compatibility means that the prefix and suffix are slightly modified from the original BioBrick prefix and suffix. Specifically, a single base pair is removed from the prefix and suffix, directly next to the part. <br> | ||
| + | The Silver prefix is: 5' GAATTC GCGGCCGC T TCTAGA 3' <Br> | ||
| + | The Silver suffix is: 5' ACTAGT A GCGGCCG CTGCAG 3' <br> | ||
| + | The scar that is formed from ligating XbaI and SpeI between two fusion compatible parts is 5' ACTAGA 3', which is six base pairs long and does not contain a stop codon. Therefore, fusion of two proteins can occur by this method. Additionally, this part is still useful and functional for non-fusion applications. There are more design considerations for when using Silver BioBrick parts. See this description by the Silver lab for more information about Silver fusion: http://www.openwetware.org/wiki/Silver:_BB_Strategy | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 15:20, 22 October 2009
Phasin (PhaP)
This is a Silver-fusion compatible BioBrick part that codes for phasin. The Silver-fusion compatibility standard was used so that this part could be fused with small signal peptides, which target proteins for export out of the cytoplasm. This part is functional both in fusion and non-fusion applications.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]