Difference between revisions of "Part:BBa K5115038"
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===Introduction=== | ===Introduction=== | ||
| − | This composite part combines [https://parts.igem.org/Part:BBa_K51150035 BBa_K51150035(ribozyme+RBS+MTA+stem-loop)],[https://parts.igem.org/Part:BBa_K51150036 BBa_K51150036(ribozyme+RBS+hpn+stem-loop)]and [https://parts.igem.org/Part:BBa_K51150033 BBa_K51150033(ribozyme+RBS+RcnR_C35L+stem-loop)] in our ribozyme-assisted polycistronic co-expression system：pRAP. | + | This composite part combines [https://parts.igem.org/Part:BBa_K51150035 BBa_K51150035(ribozyme+RBS+MTA+stem-loop)],[https://parts.igem.org/Part:BBa_K51150036 BBa_K51150036(ribozyme+RBS+hpn+stem-loop)]and [https://parts.igem.org/Part:BBa_K51150033 BBa_K51150033(ribozyme+RBS+RcnR_C35L+stem-loop)] in our ribozyme-assisted polycistronic co-expression system：pRAP. To get more information about pRAP, please check [https://2022.igem.wiki/fudan/parts part wiki of 2022 Fudan iGEM ]. |
MTA is a protein that can bind with nickel ions to reduce its toxity to the 'E.coli'. The hpn is a protein that can | MTA is a protein that can bind with nickel ions to reduce its toxity to the 'E.coli'. The hpn is a protein that can | ||
Revision as of 02:38, 29 September 2024
ribozyme connected: MTA, Hpn, RcnR_C35L
Contents
Introduction
This composite part combines BBa_K51150035(ribozyme+RBS+MTA+stem-loop),BBa_K51150036(ribozyme+RBS+hpn+stem-loop)and BBa_K51150033(ribozyme+RBS+RcnR_C35L+stem-loop) in our ribozyme-assisted polycistronic co-expression system：pRAP. To get more information about pRAP, please check part wiki of 2022 Fudan iGEM .
MTA is a protein that can bind with nickel ions to reduce its toxity to the 'E.coli'. The hpn is a protein that can sequester metals that accumulate internally to reduce nickel's toxity to the 'E.coli'. RcnR_C35L can regulate the nickel ion channel proteins in the cell membrane to tune the nickel ion transport rate.
Characterization
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 935
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 198
- 1000COMPATIBLE WITH RFC[1000]