Difference between revisions of "Part:BBa K5115059"
 
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Binding hoxU with EP can direct the hydrogenase into the carboxysome, creating a safe and stable environment for the enzyme to work.
 
Binding hoxU with EP can direct the hydrogenase into the carboxysome, creating a safe and stable environment for the enzyme to work.
  
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===Characterization===
 
  
  

Latest revision as of 01:48, 29 September 2024


hoxU fused with EP

contributed by Fudan iGEM 2023

Introduction

Combine hoxU directly with EP. The hoxU is a subunit of the hydrogenase, check it in BBa_K5115020(hox and hyp operon). EP can direct external proteins into carboxysomes, check it in BBa_K51150002(EP).

Usage and Biology

Binding hoxU with EP can direct the hydrogenase into the carboxysome, creating a safe and stable environment for the enzyme to work.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 410
    Illegal BglII site found at 488
    Illegal XhoI site found at 418
    Illegal XhoI site found at 610
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 371
    Illegal AgeI site found at 1370
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 192
    Illegal BsaI site found at 354
    Illegal SapI.rc site found at 303


References