Difference between revisions of "Part:BBa K243002:Design"
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===Source=== | ===Source=== | ||
− | + | The amino acid sequence of the DsbA signal sequence was extracted from the ''E. coli'' B genome. | |
− | Synthesized oligos | + | Synthesized oligos encoding the signal sequence of DsbA were designed and eventually ordered from Sigma. |
===References=== | ===References=== | ||
Steiner, D; Forrer, P; Stumpp, MT,(2006).Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display ''Nature Biotechnology'' Vol.24 Issue:7 Pages: 823-831 | Steiner, D; Forrer, P; Stumpp, MT,(2006).Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display ''Nature Biotechnology'' Vol.24 Issue:7 Pages: 823-831 |
Latest revision as of 02:58, 22 October 2009
DsbA signal sequence (enables periplasm export)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The signal sequence has to be located at the N-terminus of the fused protein.
Designed according to RFC 25.
Commented GenBank file
Source
The amino acid sequence of the DsbA signal sequence was extracted from the E. coli B genome. Synthesized oligos encoding the signal sequence of DsbA were designed and eventually ordered from Sigma.
References
Steiner, D; Forrer, P; Stumpp, MT,(2006).Signal sequences directing cotranslational translocation expand the range of proteins amenable to phage display Nature Biotechnology Vol.24 Issue:7 Pages: 823-831