Difference between revisions of "Part:BBa K5109060:Design"
| Line 2: | Line 2: | ||
Primer forward: 5’ – CCGCATAAGGTGCACTCTCAGTACAAT – 3’ | Primer forward: 5’ – CCGCATAAGGTGCACTCTCAGTACAAT – 3’ | ||
| + | |||
Primer reverse: 5’ – GTGCACCTTATGCGGTGTGAAATACCG – 3’ | Primer reverse: 5’ – GTGCACCTTATGCGGTGTGAAATACCG – 3’ | ||
| − | After the | + | After the mutagenesis, the restriction site was changed from CATATG to CATAAG. |
Latest revision as of 20:21, 28 September 2024
We designed primers forward and reverse overlapping the restriction site, both of them with a single mismatch on the second T of the site "CATATG".
Primer forward: 5’ – CCGCATAAGGTGCACTCTCAGTACAAT – 3’
Primer reverse: 5’ – GTGCACCTTATGCGGTGTGAAATACCG – 3’
After the mutagenesis, the restriction site was changed from CATATG to CATAAG.