Difference between revisions of "Part:BBa K079031"
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We experimentally observed that bacterial growth curve can change if cells are transformed with different genetic circuits.  Indeed, we observed that cells trasformed with BBa_K079031 and with BBa_K079032 display different growths. Moreover, we reasoned that is not sufficient to characterized a part or a device in terms of fluorescence, but is also important to understand how the bacterial cells grow and how the fluorescence expressed is related to the growth phase. For this reason, we analized both the growth and fluorescence curves in time and used them to experimentally characterize our parts and fill the experience section.
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We experimentally observed that bacterial growth curve can change if cells are transformed with different genetic circuits.  Indeed, we observed that cells trasformed with BBa_K079031 and with BBa_K079032 display different growths. Moreover, we reasoned that is not sufficient to characterized a part or a device in terms of fluorescence, but is also important to understand how the bacterial cells grow and how the fluorescence expressed is related to the growth phase.  
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For this reason, we analized both the growth and fluorescence curves in time and used them to experimentally '''characterize''' our parts and fill the '''experience''' section.
  
  

Revision as of 22:16, 21 October 2009

GFP reporter protein under the control of the BBa_J23118 promoter

GFP reporter protein under the control of the BBa_J23118 promoter

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


We experimentally observed that bacterial growth curve can change if cells are transformed with different genetic circuits. Indeed, we observed that cells trasformed with BBa_K079031 and with BBa_K079032 display different growths. Moreover, we reasoned that is not sufficient to characterized a part or a device in terms of fluorescence, but is also important to understand how the bacterial cells grow and how the fluorescence expressed is related to the growth phase. For this reason, we analized both the growth and fluorescence curves in time and used them to experimentally characterize our parts and fill the experience section.