Difference between revisions of "Part:BBa K5107006"
m
Line 5: Line 5:
 
HRE5-3WJdB is recognised by the steroid androgen, progenesterone and glycocorticoid hormone receptor.
 
HRE5-3WJdB is recognised by the steroid androgen, progenesterone and glycocorticoid hormone receptor.
  
 +
===Usage and Biology===
 +
HRE5 is used in the in cell assay as binding site of the hormones receptors. This recruitment trigger the Lac-Z expression, reporting the presence of the hormone or edc in the cell environment.<center><html><img src="https://static.igem.wiki/teams/5107/eng-incellsystem.webp"width="300" height="300"></html></center>
  
  
  
−
===Usage and Biology===
 
  
 
===Sequence and Features===
 
===Sequence and Features===

Revision as of 21:56, 28 September 2024


T7-HRE5-dB-T

HRE5-3WJdB is recognised by the steroid androgen, progenesterone and glycocorticoid hormone receptor.

Usage and Biology

HRE5 is used in the in cell assay as binding site of the hormones receptors. This recruitment trigger the Lac-Z expression, reporting the presence of the hormone or edc in the cell environment.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 72
    Illegal PstI site found at 60
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 60
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 72
    Illegal PstI site found at 60
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 72
    Illegal PstI site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]



References