Difference between revisions of "Part:BBa K5097013"
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<partinfo>BBa_K5097013 short</partinfo> | <partinfo>BBa_K5097013 short</partinfo> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
This basic part codes for an 6X Histidine tag with a N-terminal AGVG linker and C terminal double stop codon. The part is intended for use in protein purification using IMAC (Immobilized Metal ion Affinity Chromatography) purification. This part can be added to the C-terminal of a coding sequence (with the native stop codon removed), which will add the flexible AGVG linker and then a 6X Histidine tag. | This basic part codes for an 6X Histidine tag with a N-terminal AGVG linker and C terminal double stop codon. The part is intended for use in protein purification using IMAC (Immobilized Metal ion Affinity Chromatography) purification. This part can be added to the C-terminal of a coding sequence (with the native stop codon removed), which will add the flexible AGVG linker and then a 6X Histidine tag. | ||
| + | <!-- Add more about the biology of this part here | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 11:57, 30 September 2024
BBa_K5097013 6X His tag with AGVG linker
Usage and Biology
This basic part codes for an 6X Histidine tag with a N-terminal AGVG linker and C terminal double stop codon. The part is intended for use in protein purification using IMAC (Immobilized Metal ion Affinity Chromatography) purification. This part can be added to the C-terminal of a coding sequence (with the native stop codon removed), which will add the flexible AGVG linker and then a 6X Histidine tag.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]