Difference between revisions of "Part:BBa K5117037"

 
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This part serves as transcriptional unit composed of:
 
This part serves as transcriptional unit composed of:
  
−
- promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> (BBa_K5117021),
+
<ul>
 +
<li>promoter P<i>cotYZ</i> of <i>Bacillus subtilis</i> (BBa_K5117021) </i>
  
−
- ribosome binding site of <i>Bacillus subtilis</i> (BBa_K5117000),
+
<li>ribosome binding site of <i>Bacillus subtilis</i> (BBa_K5117000) </i>
  
−
- <i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4),
+
<li><i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4),
  
−
addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG (BBa_K5117025),
+
<div style="text-indent: 1px;">addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG (BBa_K5117025) </i></div>
  
−
- <i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022),
+
<li><i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022) </li>
  
−
- bidirectional terminator B0014 (BBa_B0014).
+
<li>bidirectional terminator B0014 (BBa_B0014) </li>
 +
</u>
  
  
 +
<b>Biosafety level:</b> S1
 +
 +
<b>Target organism:</b> <i>Bacillus subtilis</i>
 +
 +
<b>Main purpose of use:</b> Immobilization of BsEglS on the spore crust of <i>B. subtilis</i> (spore surface display)
 +
 +
<b>Application:</b> Degradation of cellulose
  
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
  
−
<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K5117037 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5117037 SequenceAndFeatures</partinfo>

Revision as of 15:00, 1 October 2024


PcotYZ-BsRBS-BsEglS-L3-CotY-B0014

This part serves as transcriptional unit composed of:

  • promoter PcotYZ of Bacillus subtilis (BBa_K5117021) </i>
  • ribosome binding site of Bacillus subtilis (BBa_K5117000) </i>
  • eglS gene of Bacillus subtilis without signal peptide encoding an endoglucanase (EC 3.2.1.4),
    addition of a rigid linker (L3) downstream of the coding sequence encoding the amino acids GGGEAAAKGGG (BBa_K5117025) </i>
  • cotY gene of Bacillus subtilis (BBa_K5117022)
  • bidirectional terminator B0014 (BBa_B0014)

    • </u>


    Biosafety level: S1

    Target organism: Bacillus subtilis

    Main purpose of use: Immobilization of BsEglS on the spore crust of B. subtilis (spore surface display)

    Application: Degradation of cellulose


    Sequence and Features


    Assembly Compatibility:
    • 10
      COMPATIBLE WITH RFC[10]
    • 12
      COMPATIBLE WITH RFC[12]
    • 21
      COMPATIBLE WITH RFC[21]
    • 23
      COMPATIBLE WITH RFC[23]
    • 25
      INCOMPATIBLE WITH RFC[25]
      Illegal NgoMIV site found at 1669
      Illegal AgeI site found at 726
    • 1000
      COMPATIBLE WITH RFC[1000]