Difference between revisions of "Part:BBa K5117036"
| Line 11: | Line 11: | ||
- <i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4), | - <i>eglS</i> gene of <i>Bacillus subtilis</i> without signal peptide encoding an endoglucanase (EC 3.2.1.4), | ||
| − | addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117024) | + | addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117024), |
- <i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022), | - <i>cotY</i> gene of <i>Bacillus subtilis</i> (BBa_K5117022), | ||
| − | - bidirectional terminator B0014 (BBa_B0014) | + | - bidirectional terminator B0014 (BBa_B0014). |
Revision as of 18:22, 28 September 2024
PcotYZ-BsRBS-BsEglS-L2-CotY-B0014
This part serves as transcriptional unit composed of:
- promoter PcotYZ of Bacillus subtilis (BBa_K5117021),
- ribosome binding site of Bacillus subtilis (BBa_K5117000),
- eglS gene of Bacillus subtilis without signal peptide encoding an endoglucanase (EC 3.2.1.4),
addition of a long flexible linker (L2) downstream of the coding sequence encoding the amino acids (GGGGS)4 (BBa_K5117024),
- cotY gene of Bacillus subtilis (BBa_K5117022),
- bidirectional terminator B0014 (BBa_B0014).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1696
Illegal AgeI site found at 726 - 1000COMPATIBLE WITH RFC[1000]