Difference between revisions of "Part:BBa K5269021"

Revision as of 03:45, 29 September 2024

SpyTag-ChitinaseC-His-Tag and BsmB I cut site(for GGA)

This sequence contains SD-sequence(BBa_K5269034), SpyTag, ChitinaseC (BBa_K5269014) and His-Tag. The BsmB I cleavage sites at both ends of the sequence allow for Golden Gate Assembly to the Golden Gate Assembly Site (BBa_K5269020) and T7 promoter-shRNA-T7 terminator-Golden Gate Assembly Site(BBa_K5269025). The SpyCatcher-SpyTag system works when used with Lpp-ompA-SpyCatcher(BBa_K5269026) or INPNC-SpyCatcher(BBa_K5269027). Therefore, ChitinaseC, which hydrolyzes chitin contained in the target bacterial membrane, is expected to be stably present on the Membrane Vesicle, which will facilitate the introduction of shRNA into the target fungus cells.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 7
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1022
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 7
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 7
Illegal NgoMIV site found at 150
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5269021 short</partinfo>
-This sequence contains SD sequence(BBa_K5269034), SpyTag, ChitinaseC (BBa_K5269014) and 6xHis sequence. The BsmB I cleavage sites at both ends of the sequence allow for Golden Gate Assembly to the Golden Gate Assembly Site (BBa_K5269020) and T7 promoter-shRNA-T7 terminator-Golden Gate Assembly Site(BBa_K5269025). The SpyTag/SpyCatcher system works when used with Lpp-ompA-SpyCatcher(BBa_K5269026) or INPNC-SpyCatcher(BBa_K5269027). Therefore, ChitinaseC, which hydrolyzes chitin contained in the target bacterial membrane, is expected to be stably present on the membrane vesicle, which will facilitate the introduction of shRNA into the target fungus cells.
+This sequence contains SD-sequence(BBa_K5269034), SpyTag, ChitinaseC (BBa_K5269014) and His-Tag. The BsmB I cleavage sites at both ends of the sequence allow for Golden Gate Assembly to the Golden Gate Assembly Site (BBa_K5269020) and T7 promoter-shRNA-T7 terminator-Golden Gate Assembly Site(BBa_K5269025). The SpyCatcher-SpyTag system works when used with Lpp-ompA-SpyCatcher(BBa_K5269026) or INPNC-SpyCatcher(BBa_K5269027). Therefore, ChitinaseC, which hydrolyzes chitin contained in the target bacterial membrane, is expected to be stably present on the Membrane Vesicle, which will facilitate the introduction of shRNA into the target fungus cells.
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