Difference between revisions of "Part:BBa K5269020"

Revision as of 03:43, 29 September 2024

Golden Gate Assembly Site(tac promoter, BsmB I insert, L3S2P21)

This sequence contains a tac promoter(BBa_K5269033), a BsmB I insert(BBa_K5269032), and a L3S2P21 terminator. This sequence was In-Fused to pBluescript II SK(-) In-Fusion forward primer 1 (BBa_K52690010) and pBluescript II SK(-) In-Fusion reverse primer 1 (BBa_K5269009). The two BsmB I insert(BBa_K5269032) were used for Golden Gate Assembly of the Surface displayed protein (BBa_K5269021-BBa_K5269024).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 84
Illegal BamHI site found at 189
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K5269020 short</partinfo>
-This sequence contains a tac promoter(BBa_K5269033), a BsmB I insert(BBa_K5269032), and an L3S2P21 terminator. This sequence was In-Fused to pBluescript II SK(-) In-Fusion forward primer 1 (BBa_K52690010) and pBluescript II SK(-) In-Fusion reverse primer 1 (BBa_K5269009). The two BsmB I insert(BBa_K5269032) were used for Golden Gate Assembly of the surface-presenting proteins (BBa_K5269021-BBa_K5269024).
+This sequence contains a tac promoter(BBa_K5269033), a BsmB I insert(BBa_K5269032), and a L3S2P21 terminator. This sequence was In-Fused to pBluescript II SK(-) In-Fusion forward primer 1 (BBa_K52690010) and pBluescript II SK(-) In-Fusion reverse primer 1 (BBa_K5269009). The two BsmB I insert(BBa_K5269032) were used for Golden Gate Assembly of the Surface displayed protein (BBa_K5269021-BBa_K5269024).
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